G 5000

GC analyses were performed on a G-5000 (Hitachi) equipped with a flame ionization detector with the following conditions: a fused silica capillary column (Inert Cap-Wax, 60 m × 0.25 mm, film thickness 0.25 µm, GL Sciences) and a chiral column (CP-Chirasil-Dex CB, 25 m × 0.25 mm, film thickness 0.25 µm); column temperature program: 60 ℃ for 2 min, increasing to 220 ℃ at a rate of 4 ℃/min, then held at 220℃ for 15 min; the injector was set at 60 ℃ and the detector was set at 230 ℃ [7] ; carrier gas: helium (1 ml/min); injection volume: 1 µl; split ratio: 99 : 1.
GC-MS and SPME were performed on an Agilent 6850 series gas chromatograph connected to an Agilent MSD 5975 mass spectrometer under the following operating conditions: fused silica capillary column (DB-WAX, 60 m × 0.25 mm, film thickness 0.25 µm, Agilent Technology); SPME fiber: 100 µm polydimethylsiloxane (Supelco); the column temperature program was the same as that used for the GC analysis; ionization energy: 70 eV; carrier gas: helium (1 mL/min); injection volume: 1 µl; split ratio: 99:1. The identities of most of the separated compounds were confirmed by comparison of the retention indices (RI) and mass spectra patterns (MS library: NIST 2 and flavors). RI was accordant with past data.

Agarwood oil (3 μL) and hydrosol (1 μL) samples were analyzed by GC (G-5000, Hitachi Ltd., Tokyo, Japan) equipped with a InertCap-WAX column (60 m × 0.25 mm, film thickness 0.25 μm, GL Sciences). The split ratio was 99:1. The conditions for GC analysis were as follows: carrier gas, helium; flow rate, 1 mL min -; injector temperature, 230°C; detector; flame ionization detector (FID), 250℃; column oven program, 100°C initially increased by 4°C every min until it reached 180°C, maintained at this temperature for 30 min, then increased by 5°C every min until it reached 240°C, and maintained for 18 min.