A0576

Manufactured by Agilent Technologies

Sourced in Denmark, Italy

The A0576 is a laboratory equipment product from Agilent Technologies. It is designed to perform a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

Automatically generated - may contain errors

Lab products found in correlation

A0251, by Agilent Technologies (2 mentions) Anti v5 antibody, by Thermo Fisher Scientific (1 mentions) Anti flag m2 peroxidase, by Merck Group (1 mentions) Anti na k atpase, by Thermo Fisher Scientific (1 mentions) Pt link, by Agilent Technologies (1 mentions) Envision flex target retrieval solution, by Agilent Technologies (1 mentions) Envision flex, by Agilent Technologies (1 mentions) Progres c7, by Jenoptik (1 mentions) Autostainer link, by Agilent Technologies (1 mentions) Eclipse e1000, by Nikon (1 mentions) Ab68271, by Abcam (1 mentions) Ab16659, by Abcam (1 mentions) Ab10648, by Abcam (1 mentions) Ab15580, by Abcam (1 mentions) Af3075, by R&D Systems (1 mentions) Streptavidin fitc, by Agilent Technologies (1 mentions) Biotin conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions) Bond 3, by Leica (1 mentions)

4 protocols using a0576

Antibody Panel for Cell Signaling Analysis

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In this study, the following antibodies were used: Anti-thyroglobulin (M0781, Dako), anti-calcitonin (A0576, Dako) anti-phospho-tyrosine (P-Tyr-1000) MultiMab™ (8954S, CST), anti-Myelin Basic Protein (MBP) (13344, CST), anti-V5 antibody (Thermo Fisher Scientific, R960-25), anti-FLAG® M2-Peroxidase (A8592, Sigma), anti-phospho-RET (Y905) (3221S, CST), anti-RET (C31B4) (3223S, CST), anti-phospho-STAT3 (Ser727) (9134P, CST), anti-STAT3 (9139P, CST), anti-phospho-Akt (Thr308) (9275, CST), anti-Akt (C67E7) (4691, CST), anti-phospho-MEK1/2 (9154, CST), anti-MEK1 antibody (2352, CST), anti-phospho-p44/42 MAPK (Thr202/Tyr204) (ERK1/2) (9101L, CST), anti-p44/42 MAPK (ERK1/2) (9102, CST), anti-M2-PK (S-1, Schebo Biotech), anti-histone H3 (4499S, CST), anti-actin (ab49900, CST), anti-Na-K ATPase (MA3-928, Thermo Fisher Scientific), anti-HUWE1 (A300-486A, Bethyl), anti-USP7 (A300-033A, Bethyl), anti-USP9X (A301-351A, Bethyl) and anti-KRAS (c-166691, Santa Cruz).

Krishnan A., Berthelet J., Renaud E., Rosigkeit S., Distler U., Stawiski E., Wang J., Modrusan Z., Fiedler M., Bienz M., Tenzer S., Schad A., Roth W., Thiede B., Seshagiri S., Musholt T.J, & Rajalingam K. (2020). Proteogenomics analysis unveils a TFG-RET gene fusion and druggable targets in papillary thyroid carcinomas. Nature Communications, 11, 2056.

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Immunohistochemical Characterization of MTC Tumours

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The formalin-fixed tumours were dehydrated, embedded in paraffin, and sliced into sections of 4 μm, according to standard procedures. To verify the MTC origin of the tumours, sections from the mock-treated control group were stained with haematoxylin and eosin (H&E) for morphological examination, and by using antibodies for MTC markers chromogranin A (CgA, dilution 1:500; ab68271, Abcam, Cambridge, England), synaptophysin (Syn, dilution 1:25; ab16659, Abcam), and calcitonin (Ctn, dilution 1:1000; A0576, Dako, Glostrup, Denmark). Antibodies were incubated for 1 hour.
For the immunohistochemical (IHC) staining, tumour sections were collected on glass slides and then treated with EnVision^™ FLEX Target Retrieval Solution (high pH) using a PT-Link (Dako). The staining was done in an Autostainer Link using EnVision^™ FLEX (Dako) according to the manufacturer’s instructions. Positive and negative controls were included in each run. A microscope (20x magnification, Eclipse E1000, Nikon Instruments, Amsterdam, Netherlands) equipped with a camera (ProgRes C7, Jenoptik, Jena, Germany) was used for imaging of the stained tumour sections.

Sandblom V., Spetz J., Shubbar E., Montelius M., Ståhl I., Swanpalmer J., Nilsson O, & Forssell-Aronsson E. (2020). Increased therapeutic effect on medullary thyroid cancer using a combination of radiation and tyrosine kinase inhibitors. PLoS ONE, 15(5), e0233720.

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Immunofluorescence Analysis of Thyroid Markers

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Primary antibodies used for immunofluorescence were: rabbit anti-Nkx2-1 (1:1000; PA0100, EAtlab srls); rabbit anti-Fgf10 (1:500; ABN44, EMD Millipore); rabbit anti-Fgfr2 (1:5000; ab10648, Abcam); goat anti-hSOX9 (1:500; AF3075, R&D Systems); rat anti-E-cadherin (ECCD-2; 1:500; 205604, Calbiochem); rabbit anti-Ki-67 (1:100; ab15580, Abcam); rabbit anti-Pax8 (kindly provided by Roberto di Lauro, Universita di Napoli Federico II, Naples, Italy); rabbit anti-Tg (1:3000; A0251, Dako); rabbit anti-calcitonin (1:500; A0576, Dako). Secondary antibodies used include: Rhodamine Red-X-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories); Rhodamine Red-X-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories); biotin-conjugated donkey anti-rat IgG (Jackson ImmunoResearch Laboratories) and biotin-conjugated donkey anti-goat IgG (Jackson ImmunoResearch Laboratories) followed by Streptavidin-FITC (Dako).

Liang S., Johansson E., Barila G., Altschuler D.L., Fagman H, & Nilsson M. (2018). A branching morphogenesis program governs embryonic growth of the thyroid gland. Development (Cambridge, England), 145(2), dev146829.

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Immunohistochemical Analysis of Thyroid Markers

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Tumor tissues were fixed overnight in 10% buffered formalin and embedded in paraffin. Each section was stained with hematoxylin and eosin (H&E). Unstained paraffin sections were deparaffinized and immunostained using Leica Bond III (Leica Biosystems, Wetzlar, Germany) according to the manufacturer's protocol. Anti-calcitonin antibody (A0576, Dako, 1:500) and antithyroglobulin antibody (A0251, Dako, 1:200) were used as primary antibodies.

Takeshima K., Ariyasu H., Uraki S., Morita S., Furukawa Y., Inaba H., Iwakura H., Doi A., Warigaya K., Murata S.I., Enomoto K., Hotomi M, & Akamizu T. (2020). False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization. Endocrine journal, 67(10).

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