HEK-293A cells were transfected with GAL-hAR-658-919 (Addgene, Cat# 89082), UAS-promoter Firefly luciferase and Renilla luciferase reporter plasmids. After 24 h incubation, 100 nM 5α-Androstan-17β-ol-3-one (DHT, Sigma, Cat# A8380) were treated with or without different concentrations of neuroprotection library compounds (InterPham) or pinostilbene hydrate (Sigma, Cat# SML0098) for 12 h.
Pinostilbene hydrate
Manufactured by Merck Group
Sourced in United States
Pinostilbene hydrate is a white crystalline solid chemical compound. It is used as a laboratory reagent for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
5α androstan 17β ol 3 one dht, by Merck Group (1 mentions)
Phospho akt, by Cell Signaling Technology (1 mentions)
Pd98059, by Thermo Fisher Scientific (1 mentions)
L dopa, by Merck Group (1 mentions)
Phospho erk, by Cell Signaling Technology (1 mentions)
α msh, by Merck Group (1 mentions)
Enhanced chemiluminescence ecl kit, by Biosesang (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Bicinchoninic acid bca assay kit, by Thermo Fisher Scientific (1 mentions)
Antibodies against tyrosinase, by Santa Cruz Biotechnology (1 mentions)
Laemmli sample buffer, by Biosesang (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Sp600125, by Santa Cruz Biotechnology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
β actin, by Novus Biologicals (1 mentions)
U0126, by Santa Cruz Biotechnology (1 mentions)
Claudin 1, by Cell Signaling Technology (1 mentions)
4 protocols using pinostilbene hydrate
1
Neuroprotection Screening of Compounds in HEK-293A Cells
2
Pigmentation Regulation in Melanocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pinostilbene hydrate, dimethyl sulfoxide (DMSO), α-MSH, NaOH, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), radioimmunoprecipitation assay (RIPA) buffer, protease inhibitor cocktail, and L-DOPA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin/streptomycin (P/S), trypsin–ethylenediaminetetraacetic acid (T/E), bicinchoninic acid assay (BCA) kit, and PD98059, an ERK inhibitor, were obtained from Thermo Fisher Scientific (Waltham, MA, USA). Antibodies against tyrosinase, TRP-1, TRP-2, and MITF were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibodies against phospho-p38, p38, phospho-JNK, JNK, phospho-ERK, ERK, phospho-AKT, AKT, and β-actin were obtained from Cell Signaling Technology (Danvers, MA, USA). An enhanced chemiluminescence (ECL) kit, phosphate-buffered saline (PBS), radioimmunoprecipitation assay (RIPA) buffer, and 2× Laemmli sample buffer were obtained from Biosesang (Sungnam, Gyeonggi-do, Korea) and Bio-Rad (Hercules, CA, USA), respectively.
3
Pinostilbene Hydrate Inhibits EMT Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Pinostilbene hydrate (3, 4′-Dihydroxy-5-methoxy-trans-stilbene hydrate, PSH) (≥95% purity) was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in pure grade dimethyl sulfoxide (DMSO). Antibodies against MMP-2, protein kinase B (PKB, also known as AKT), phospho-AKT (p-AKT), p38, phospho-p38 (p-p38), extracellular signal-regulated kinase 1/2 (ERK 1/2), phospho-ERK 1/2 (p-ERK1/2), c-Jun N-terminal kinase 1/2 (JNK 1/2), phospho-JNK1/2 (p-JNK1/2), E-cadherin, Claudin-1, Vimentin, and N-cadherin were purchased from cell Signaling Technology (Danvers, MA, USA) and stored at −20°C. β-actin was bought from Novus Biologicals (Littleton, CO, USA). 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich (St Louis, MO, USA) and stored at 4°C. Specific ERK1/2 inhibitor (U0126) and JNK1/2 inhibitor (SP600125) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and stored at −20°C.
4
Comprehensive Analytical Techniques for Phenolic Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals used were of analytical reagent grade. All reagents were purchased from Sigma, Aldrich: Ascorbic acid, Acetic acid, acetylcholinesterase (AChE), acetylthiocholine (ACTHI) acetonitrile (ACN), N, O-bis (trimethylsilyl) trifluoroacetamide (BSTFA), cyclohexane (CYH), dichloromethane (DCM), Dulbecco's modified eagle medium (DMEM), dimethyl sulfoxide (DMSO), 1-1-diphenyl-2-picryl hydrazyl (DPPH), 5, 5-dithiobis-2-nitrobenzoic acid (DTNB), Folin-Ciocalteu reagent (2 N), methanol (MeOH), human colon cancer cells (HCT116), human breast cancer cells (MCF-7), 3- [4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide (MTT), Roswell Park Memorial Institute (RPMI), Chlorotrimethylsilane (TMCS), and tetrahydrofuran (THF).
The analytical standards used for the identification and quantification of the principal phenolic compounds found in the extracts: 3-amino-4-hydroxybenzoic acid, Gallic acid, 3, 4-dihydroxy-5 methoxybenzoic acid, 7-hydroxycoumarin-3-carboxylic acid, rutin hydrate, Butyl gallate, 4-hydroxytamoxifen, cardamonin, phenoxodiol, pinostilbene hydrate, 3-benzyloxy-4, 5-dihydroxy-benzoic acid methyl ester, ethyl trans-2-hydroxycinnamate, 4′, 5-dihydroxy-7-methoxyflavone, pinosylvin monomethyl ether, 3, 6, 3′-trimethoxyflavone, shikonin, 5-hydroxy-3′-methoxyflavone, and 3′-hydroxy-b-naphthoflavone were also purchased from Sigma, Aldrich.
The analytical standards used for the identification and quantification of the principal phenolic compounds found in the extracts: 3-amino-4-hydroxybenzoic acid, Gallic acid, 3, 4-dihydroxy-5 methoxybenzoic acid, 7-hydroxycoumarin-3-carboxylic acid, rutin hydrate, Butyl gallate, 4-hydroxytamoxifen, cardamonin, phenoxodiol, pinostilbene hydrate, 3-benzyloxy-4, 5-dihydroxy-benzoic acid methyl ester, ethyl trans-2-hydroxycinnamate, 4′, 5-dihydroxy-7-methoxyflavone, pinosylvin monomethyl ether, 3, 6, 3′-trimethoxyflavone, shikonin, 5-hydroxy-3′-methoxyflavone, and 3′-hydroxy-b-naphthoflavone were also purchased from Sigma, Aldrich.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!