For analyses of the SMs, strains were grown in submerged cultures as described above. After 7 days, mycelia were removed from the culture by filtration through Mirachloth (Calbiochem, Merck KGaA, Darmstadt, Germany). Small particulates were removed from the culture filtrates using 0.45 μm syringe filters (BGB®, Schloßböckelheim, Germany) which were then analyzed by high-pressure liquid chromatography with a diode array detector [HPLC-DAD; Hitachi Chromaster LC equipped with a 250 mm × 4.60 mm i.d., 5 μm, Gemini® C18 with a 4 mm × 3 mm Gemini® C18 guard column (Phenomenex, Aschaffenburg, Germany)], a with 5160 pump, 5260 autosampler, 5310 column oven, and 5430 DAD (VWR International GmbH, Darmstadt, Germany). For data analyses, the software EZChrom Elite (VWR, Darmstadt, Germany) was used.
5260 autosampler
Manufactured by Avantor
Sourced in Germany
The 5260 autosampler is a laboratory instrument designed to automatically introduce samples into an analytical system, such as a gas chromatograph or liquid chromatograph. It offers precision and consistency in sample handling, helping to improve the reliability and efficiency of analytical workflows.
Automatically generated - may contain errors
Lab products found in correlation
5160 pump, by Avantor (2 mentions)
5310 column oven, by Avantor (2 mentions)
Gemini c18, by Phenomenex (1 mentions)
Ezchrom elite, by Avantor (1 mentions)
Gemini c18 guard column, by Phenomenex (1 mentions)
Openlab control panel software, by Agilent Technologies (1 mentions)
Liquid chromatograph, by Hitachi (1 mentions)
2 protocols using 5260 autosampler
1
Analysis of Secondary Metabolites via HPLC-DAD
2
HPLC and UPLC-ESI-QTOF-MS Analysis of Hyperforins and Hypericins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The HPLC system consisted of a VWR
HITACHI liquid chromatograph equipped with a 5160 pump, a 5260 autosampler,
a 5430 diode array detector, an organizer (eluent stand), and temperature
controller for the column and sampler (5310 column oven) (VWR, HITACHI,
MA, JAPAN). The absorption was measured in the range of 200–800
nm, with the UV–visible detector working at 260 nm for hyperforins
and 350 and 590 nm for hypericins. The chromatographic data were recorded
and processed with Agilent Open LAB Control Panel software. For UPLC-ESI-QTOF-MS
analysis, a Waters Acquity high-performance liquid chromatograph (UPLC,
Waters) coupled with a time-of-flight Q-TOF micro-mass spectrometer
(Waters) and equipped with electrospray ionization (ESI) was used.
Mass spectra were recorded in the positive ion mode at 3 kV capillary
voltage and 230 °C desolvation temperature.
HITACHI liquid chromatograph equipped with a 5160 pump, a 5260 autosampler,
a 5430 diode array detector, an organizer (eluent stand), and temperature
controller for the column and sampler (5310 column oven) (VWR, HITACHI,
MA, JAPAN). The absorption was measured in the range of 200–800
nm, with the UV–visible detector working at 260 nm for hyperforins
and 350 and 590 nm for hypericins. The chromatographic data were recorded
and processed with Agilent Open LAB Control Panel software. For UPLC-ESI-QTOF-MS
analysis, a Waters Acquity high-performance liquid chromatograph (UPLC,
Waters) coupled with a time-of-flight Q-TOF micro-mass spectrometer
(Waters) and equipped with electrospray ionization (ESI) was used.
Mass spectra were recorded in the positive ion mode at 3 kV capillary
voltage and 230 °C desolvation temperature.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!