Rabbit anti-LC3A/B (4108), anti-p-AKT (Ser473) (4060), anti-AKT (4691), and anti-p-MTOR (Ser2448; 5536), anti-MTOR (2983), anti-p-PRKAA (4185), anti-PRKAA (2532), anti-p-MAPK1/3 (4370), anti-MAPK1/3 (4695), anti-p-MAPK11 (4511), and anti-MAPK11 (9212), NBR1 (9891) antibodies were purchased from Cell Signaling Technology. Rabbit anti-CASP2 (ab179520), anti-PIK3C3 (ab124095), anti-p-ULK1 (ab133747), anti-ULK1 (ab128859), anti-CASP3 (ab179517), anti-CASP8 (ab25901), anti-CASP9 (ab2014) and anti-ATG5 (ab108327) antibodies were purchased from Abcam. Anti-BECN1 (sc-48341) antibody was purchased from Santa Cruz Biotechnology. Rabbit anti-SQSTM1 (3340–1), anti-MYC (R1208–1) and anti-GAPDH/glyceraldehyde-3-phosphate dehydrogenase (2251–1) antibodies were purchased from Epitomics. Mouse monoclonal antibodies (mAbs) to N and P proteins of RABV were produced in our laboratory.57 (link) Dulbecco's modified Eagle's medium (DMEM, 11995), Donkey anti-mouse IgG secondary antibody, Alexa Fluor® 647 (A-31571) and 546 (A10036) conjugate were obtained from Thermo Scientific. Rapa (R8781), wortmannin (W1628) and 3-MA (M9281) were purchased from Sigma-Aldrich. EBSS (14155–063) was purchased from Gibco. CQ (50–63–5[tlrl-chq]) was purchased from Invivogen. EXFectTM Transfection Reagent (T101–01/02) was purchased from Vazyme Biotech and LysoTracker Red DND-99 (L-7528) was purchased from Invitrogen.
Anti atg5 ab108327
Manufactured by Abcam
Sourced in United States
Anti-ATG5 (ab108327) is a primary antibody that recognizes the ATG5 protein. ATG5 is a key component of the autophagy process and is involved in the formation of autophagosomes. This antibody can be used to detect and study the expression of ATG5 in various cell and tissue types.
Automatically generated - may contain errors
2 protocols using anti atg5 ab108327
1
Autophagy and Cell Signaling Pathway Analysis
2
Antibody-based Immunoblotting Protocol
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Rabbit anti-LC3A/B (4108), anti-p-AKT (Ser473) (4060), anti-AKT (4691), and anti-p-MTOR (Ser2448; 5536), anti-MTOR (2983), antipAMPK (4185), anti-AMPK (2532), anti-p-ERK (4370), anti-ERK (4695), antip-P38 (4511), and anti-P38 (9212) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-P62 (3340-1) antibodies were purchased from Epitomics (Burlingame, CA, USA). Rabbit anti-CASP2 (ab179520), anti-ULK1 (ab128859), and anti-ATG5 (ab108327) antibodies were purchased from Abcam (Cambridge, MA, USA). Mouse anti-ATG7 (sc-376212) and anti-BECN1 (sc-48341) antibodies was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Mouse anti-Flag (clone M2) (F1804) mAb, wortmannin (W1628), and 3-MA (M9281) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-MYC (R12081) and anti-GAPDH/glyceraldehyde-3-phosphate dehydrogenase (EM1101) antibodies were purchased from Huaan Biological Technology (Hangzhou, China). Mouse monoclonal antibodies (mAbs) recognizing theN/P proteinsof RABV were produced in our laboratory [15] .
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