WT or SCD mice were treated with Ampicillin (1g/L), Streptomycin (1g/L), Metronidazol (1g/L) and Vancomycin (1g/L) in drinking water for 4 – 6 weeks. Antibiotics were purchased from Sigma or Jack D. Weiler Hospital of the Albert Einstein College of Medicine. Drinking water containing antibiotics was changed every 3 – 4 days. For microbial product add-back experiment, ABX-treated WT mice were fed with 1 mg LPS (0111:B4, Sigma), 1 mg Peptidoglycan (PGN-SA, Invivogen) or 1mg MurNAc-L-Ala-γ-D-Glu-mDAP (M-TriDAP) by intragastric gavage, and allowed to rest for 24 – 36 h. For the analysis of neutrophil-LPS interaction, untreated WT mice were fed with 300 μg LPS-FITC (Sigma) by intragastric gavage, and tissues were harvested 1 hour after gavage.
Peptidoglycan pgn sa
Manufactured by InvivoGen
Peptidoglycan (PGN-SA) is a structural component of the cell wall of Gram-positive bacteria. It functions as a pathogen-associated molecular pattern (PAMP) that can be recognized by pattern recognition receptors (PRRs) in the host immune system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using peptidoglycan pgn sa
1
Antibiotic-Induced Gut Modulation
2
Antibiotic-Induced Gut Modulation
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WT or SCD mice were treated with Ampicillin (1g/L), Streptomycin (1g/L), Metronidazol (1g/L) and Vancomycin (1g/L) in drinking water for 4 – 6 weeks. Antibiotics were purchased from Sigma or Jack D. Weiler Hospital of the Albert Einstein College of Medicine. Drinking water containing antibiotics was changed every 3 – 4 days. For microbial product add-back experiment, ABX-treated WT mice were fed with 1 mg LPS (0111:B4, Sigma), 1 mg Peptidoglycan (PGN-SA, Invivogen) or 1mg MurNAc-L-Ala-γ-D-Glu-mDAP (M-TriDAP) by intragastric gavage, and allowed to rest for 24 – 36 h. For the analysis of neutrophil-LPS interaction, untreated WT mice were fed with 300 μg LPS-FITC (Sigma) by intragastric gavage, and tissues were harvested 1 hour after gavage.
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