Iron 3 hexahydrate

Tannic acid (TA, ACS reagent), titanium (IV) bis (ammonium lactato) dihydroxide (Ti-BALDH) solutions 50 wt% in H₂O, iron (III) hexahydrate (FeCl₃.6H₂O), copper (II) chloride (CuCl₂), cobalt (II) bromide (CoBr₂), nickel (II) chloride (NiCl₂), zinc (II) chloride (ZnCl₂), 2,6-dichlorophenolindophenol, trizma hydrochloride (Tris), copper assay kit and peroxidase from horseradish (HRP) lyophilized powder were purchased from Sigma-Aldrich. Phosphate buffer saline (PBS) was obtained from Acros Organics. Luria-Bertani (LB) and tryptic soy broth (TSB) were purchased from Gibco. MTT assay kit and sodium hydroxide (NaOH) were acquired from Thermo Fisher Scientific. Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco. NIH 3T3 fibroblasts were obtained from Bioresource Collections and Research Center (BCRC), Taiwan. S. epidermidis ATCC12228, methicillin-resistant S. aureus (USA300, ATCC BAA-1718) and E. coli ATCC12435 were acquired from Bioresource Collections and Research Center in Taiwan. All the water used was purified to 18.2 mΩ using a Millipore water purifications system, and filtered using a 0.22 µm filter.

The reagents used were iron (III) hexahydrate (FeCl₃·6H₂O) ≥ 98% trademark purity Sigma-Aldrich (St. Louis, MO, USA); iron (II) chloride tetrahydrate (FeCl₂·4H₂O) ≥ 98% trademark purity Sigma-Aldrich (St. Louis, MO, USA); ammonium hydroxide solution at 30% purity of the commercial brand LABQUIMAR (San Jose, Costa Rica); absolute ethyl alcohol of the trademark J.T. Baker (Madrid, Spain); and toluene of the trademark J.T. Baker (Madrid, Spain), all distributed by Industrial Casjim Costa Rica.
Sapwood from Pinus oocarpa, Vochysia ferruginea and Vochysia guatemalensis wood from fast-growing forest plantations in Costa Rica was used, which has been studied and showed good liquid permeability [26 (link)] and has also been tested due to its adequate absorption of different substances in the treatments toward the improvement of its properties [26 (link),27 ,36 (link)]. It is important to clarify that in this article, the magnetic permeability was not investigated; therefore, the permeability indicated in the article refers only to liquid permeability. Twenty selected sapwood boards were dried in a moisture content (MC) between 12–15%, from which samples of 6 cm long × 3 cm wide and 2 cm thick were prepared.