SK-N-BE (2) cells (ATCC) were cultured in Minimum Essential Medium supplemented with 100 nM L-glutamine, 1% penicillin-streptomycin, 1% 1 : 1 nonessential amino acid solution: Ham's F-12, and 15% fetal bovine serum (FBS). IMR-32 cells (ATCC) were cultured in Minimum Essential Medium supplemented with 100 nM L-glutamine, 1% penicillin-streptomycin, 1% nonessential amino acid solution, and 10% FBS. SH-SY5Y cells (ATCC) were cultured in Dulbecco's modified Eagle's medium (DMEM)/Nutrient Mixture F-12 Ham's supplemented with 100 nM L-glutamine, 1% penicillin-streptomycin, and 10% FBS. HEK293T cells were cultured in high-glucose DMEM supplemented with 100 nM L-glutamine, 1% penicillin-streptomycin, and 10% FBS (all from Sigma). All cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
Sk n be 2 cells
Manufactured by American Type Culture Collection
Sourced in United States, Germany
SK-N-BE(2) cells are a commonly used human cell line derived from a neuroblastoma tumor. They exhibit characteristics of neuronal cells and are commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Sh sy5y cell, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Sk n fi, by Leibniz Institute DSMZ (2 mentions)
Imr 5 75, by Leibniz Institute DSMZ (2 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Imr 32 cells, by American Type Culture Collection (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
Mcf 7 cell, by American Type Culture Collection (1 mentions)
Mda mb 231 cell, by American Type Culture Collection (1 mentions)
Du145 cells, by American Type Culture Collection (1 mentions)
4t1 cell, by American Type Culture Collection (1 mentions)
Bt474 cells, by American Type Culture Collection (1 mentions)
Mda mb 453 cells, by American Type Culture Collection (1 mentions)
Mda mb 436 cells, by American Type Culture Collection (1 mentions)
Rat c6 glioma cells, by American Type Culture Collection (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Insulin transferrin selenium g supplement, by Thermo Fisher Scientific (1 mentions)
8 well cell culture slide, by SPL Life Sciences (1 mentions)
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)
6 protocols using sk n be 2 cells
1
Culturing Neuroblastoma and HEK293T Cells
2
Culturing SK-N-BE(2) Cells for Research
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Human SK-N-BE(2) cells were purchased from ATCC. The cells were maintained at 37°C and 5% CO2 in 1:1 mixture of ATCC-formulated Eagle’s Minimum Essential Medium (EMEM) and F-12 K medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin.
3
Caspase-8 Modulates Neuroblastoma Cell Death
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Caspase-8-defective human neuroblastoma SK-N-BE(2) cells (ATCC® CRL-2271) are available at ATCC (CRL-2271) and were obtained from Marie A. Henriksson (Karolinska Institutet) and tested negative for mycoplasma contamination. Cells were maintained at 37°C, 5% CO₂, in DMEM/F12 medium (Gibco BRL) supplemented with 10% heat-inactivated fetal bovine serum, 100U/ml penicillin and 100μg/ml streptomycin. Cells were seeded at 75,000 cells/well in 12 well plates 24 h prior to transfection. 2μl Lipofectamine 2000 were used together with 0.5μg (Figs 1 and 2 , S1 Fig S1 File
4
Cell Culture Conditions for Diverse Cell Lines
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C6 rat glioma cells (ATCC #CCL-107), HEK293 cells (ATCC #CRL-1573), GT1-7 cells [62 (link)], CLU188 cells [63 (link)], SH-SY5Y cells (ATCC #CRL-2266), SK-N-BE2 cells (ATCC #CRL-2271), iNHA cells [63 (link)], MDA-MB-231 cells (ATCC #HTB-26), MDA-MB-436 cells (ATCC #HTB-130), MDA-MB-453 cells (ATCC #HTB-131), MCF7 cells (ATCC #HTB-22), 4T1 cells (ATCC #CRL-2539), BT474 cells (ATCC #HTB-20), CHO cells (ATCC #CCL-61), OVK18 cells [64 (link)], DU145 cells (ATCC #HTB-81), and HCCLM3 cells [65 (link)] were maintained as a monolayer culture on tissue culture dishes at 37 °C, 5% CO2, 100% humidity in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum and antibiotics. SH-SY5Y cells were differentiated in the presence of 10 μM retinoic acid for 1 week [66 (link)]. OC-k3 cells [67 (link)] were maintained as a monolayer on tissue culture dishes at 33 °C, 10% CO2, 100% humidity in DMEM supplemented with 10% heat-inactivated fetal bovine serum, 50 U/mL of recombinant mouse interferon-γ, and antibiotics. S1P and CYM-5478 were solubilized with bovine serum albumin (0.1% final concentration) prior to treatment.
5
Bioprinting of SK-N-BE(2) Neuroblastoma Cells
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SK-N-BE(2) cells were acquired from American Type Culture Collection (ATCC, Manassas, VA, USA) and expanded in a growth medium based on Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco, Thermofisher), supplemented with 10% fetal bovine serum (Thermofisher), 1% Insulin-Transferrin-Selenium G Supplement (Thermofisher), Plasmocin (0.2%) treatment ant-mpt (1/10) (InvivoGen) and 1% penicillin/streptomycin (Thermofisher) at 37 °C and 5% CO2 atmosphere. 2D cell cultures were grown in 8-well Cell Culture Slides (SPL Life Sciences) until they reached confluence before immunocytochemistry (ICC) analysis.
To create the bioinks, cells were cultured and trypsinized. The resulting pellet was resuspended with the prepolymer solution at 37 °C to a 2.5 × 106 cell density. The bioink was loaded in a bioprinting syringe and gelified at −20 °C for 3 minutes before printing.
To create the bioinks, cells were cultured and trypsinized. The resulting pellet was resuspended with the prepolymer solution at 37 °C to a 2.5 × 106 cell density. The bioink was loaded in a bioprinting syringe and gelified at −20 °C for 3 minutes before printing.
6
Neuroblastoma Cell Lines Culture Protocol
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The human neuroblastoma cells lines, NB69, SK-N-FI and IMR-5/75, were purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen (Braunschweig, Germany) and cultured in Roswell Park Memorial Institute (RPMI) 1640 media (GIBCO) supplemented with 10% fetal calf serum. SK-N-BE(2) cells were purchased from the American Type Culture Collection (Wesel, Germany), and cultured in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO) supplemented with 10% fetal calf serum. The NXS2 murine neuroblastoma cell line19 (link) was kindly provided by Holger N. Lode (University Medicine Greifswald, Germany) and cultured in DMEM supplemented with 10% fetal calf serum, 100 U/mL penicillin and 100 µg/mL streptomycin. All cell lines were cultured at 37°C in 5% CO2. Cell line identity was assured by short tandem repeat DNA genotyping. Cultures were routinely tested for mycoplasma using the PlasmoTest Kit (Invitrogen).
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