The experiment was performed on an FEI Tecnai 12 G2 Spirit TWIN TEM as described in [29 (link)]. A 3μLdrop of the sample (liposomes containing CCCTAA) was applied to a glow-discharged TEM grid (300-mesh Cu grid) coated with a holey carbon film (Lacey substrate, Ted Pella, Ltd., Redding, CA, USA). The excess liquid was blotted, and the specimen was vitrified by a rapid plunging into liquid ethane pre-cooled with liquid nitrogen using Vitrobot Mark IV (FEI). The vitrified samples were then transferred to a cryo-holder (Gatan model 626) and examined at −177 °C using a FEI Tecnai 12 G2Spirit TWIN TEM. The images were recorded by a 4K × 4K (FEI Eagle) CCD camera at 120 kV in low-dose mode. TIA (Tecnai Imaging & Analysis, Weinheim, Germany) software was used to record the images.
Tecnai12 g2 spirit twin tem
Manufactured by Thermo Fisher Scientific
The Tecnai12 G2 Spirit TWIN TEM is a transmission electron microscope (TEM) designed for high-resolution imaging and analysis of various materials. It features a twin-lens configuration that provides enhanced flexibility and performance. The core function of this product is to enable researchers and scientists to visualize and study the microstructure and composition of samples at the nanoscale level.
Automatically generated - may contain errors
3 protocols using tecnai12 g2 spirit twin tem
1
Cryogenic TEM Imaging of Liposomes
2
Cryo-EM Visualization of Protein Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Three representative samples, A3, B3, and C6, and two references,
keratin and PLD, were tested. Samples, excluding PLD, were prepared
at the following procedure: a 3 μL drop of the sample was applied
to a glow-discharged TEM grid (300-mesh Cu grid) coated with a holey
carbon film (Lacey substrate, Ted Pella, Ltd.). The excess liquid
was blotted, and the specimen was vitrified by rapidly plunging into
liquid ethane precooled with liquid nitrogen using Vitrobot Mark IV
(FEI). The vitrified samples were transferred to a cryo-holder (Gatan
model 626) and examined at −177 °C using an FEI Tecnai
12 G2 Spirit TWIN TEM. The images were recorded by a 4 K × 4
K Eagle CCD camera (FEI) at 120 kV in low-dose mode. TEM imaging was
applied for PLD, and a 3 μL drop was applied to a glow-discharged
TEM grid (carbon-supported film on 300-mesh Cu grids, Ted Pella).
After 30 s, the excess liquid was blotted, and the grids were allowed
to dry for 2 h at room temperature before they were observed by an
FEI Tecnai 12 G2 TWIN TEM operated at 120 kV. The images were recorded
by a 4 K × 4 K FEI Eagle CCD camera.
keratin and PLD, were tested. Samples, excluding PLD, were prepared
at the following procedure: a 3 μL drop of the sample was applied
to a glow-discharged TEM grid (300-mesh Cu grid) coated with a holey
carbon film (Lacey substrate, Ted Pella, Ltd.). The excess liquid
was blotted, and the specimen was vitrified by rapidly plunging into
liquid ethane precooled with liquid nitrogen using Vitrobot Mark IV
(FEI). The vitrified samples were transferred to a cryo-holder (Gatan
model 626) and examined at −177 °C using an FEI Tecnai
12 G2 Spirit TWIN TEM. The images were recorded by a 4 K × 4
K Eagle CCD camera (FEI) at 120 kV in low-dose mode. TEM imaging was
applied for PLD, and a 3 μL drop was applied to a glow-discharged
TEM grid (carbon-supported film on 300-mesh Cu grids, Ted Pella).
After 30 s, the excess liquid was blotted, and the grids were allowed
to dry for 2 h at room temperature before they were observed by an
FEI Tecnai 12 G2 TWIN TEM operated at 120 kV. The images were recorded
by a 4 K × 4 K FEI Eagle CCD camera.
3
Cryo-TEM Imaging of Liposomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
А drop (3.5 μl) of a liposome suspension was applied to a glow discharged TEM grid (300-mesh Cu grid) coated with a holey carbon film (Lacey substrate, Ted Pella, Ltd.). The excess liquid was blotted, and the specimen was vitrified by a rapid plunging into liquid ethane pre-cooled with liquid nitrogen using Vitrobot Mark IV (FEI). The vitrified samples were transferred to a cryo-holder (Gatan model 626), and examined at -177 C using a FEI Tecnai 12 G 2 Spirit TWIN TEM. The images were recorded (4Kx4K FEI Eagle CCD camera) at 120 kV in low-dose mode. TIA (Tecnai Imaging & Analysis) software was used to record the images and to measure the thickness of the liposome's membrane.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!