HepG2, HepG2-BMP4, SMMC7721, and SMMC7721-shBMP4 cells were lysed in 10% SDS. Cell lysates were first subject to SDS-PAGE, transferred to polyvinylidene fluoride membranes (Millipore, USA) and then blocked in non-fat milk. Primary antibodies including BMP4 (ab124715, Abcam, USA), E-cadherin (zs-78700, Zhongshan Chemical Co., China), Vimentin (2707-1, Epitomics, USA), EphA2 (sc-924, Santa Cruz Biotechnology, USA), VE-cadherin (ab33168, Abcam, USA), MMP2 (sc-13595, Santa Cruz Biotechnology, USA), OCT4 (sc-8629, Santa Cruz Biotechnology, USA) and SOX2 (GTX101507, GeneTex, USA) were added to the membranes at 4°C overnight. The next day, the secondary antibodies including goat anti-rabbit IgG-HRP (ZB-2301, Santa Cruz Biotechnology, USA), goat anti-mouse IgG-HRP (ZB-2305, Zhongshan Chemical Co., China) and rabbit anti-goat IgG-HRP (ZB-2306, Zhongshan Chemical Co., China) were added for 2 h at 37°C. The protein levels were assessed using the enhanced chemiluminescence method.
Zb 2301
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The ZB-2301 is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 6,000 RPM and a maximum RCF of 3,500 x g. The centrifuge can accommodate a variety of rotor types and sample volumes.
Automatically generated - may contain errors
Lab products found in correlation
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
Vimentin, by Abcam (1 mentions)
Ab124715, by Abcam (1 mentions)
Mmp 2, by Santa Cruz Biotechnology (1 mentions)
Epha2, by Santa Cruz Biotechnology (1 mentions)
Ve cadherin, by Abcam (1 mentions)
Ab50010, by Abcam (1 mentions)
Ab3525, by Abcam (1 mentions)
Total protein extraction kit, by Applygen (1 mentions)
β actin, by Immunoway (1 mentions)
2 protocols using zb 2301
1
Comprehensive Protein Expression Analysis
2
Hippocampal Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The hippocampi of 5 rats of each group were dissected, weighed, recorded and snap frozen in liquid nitrogen immediately after finishing the MWM test. Proteins were extracted with an Applygen total protein extraction kit. The protein concentration was normalized using Coomassie brilliant blue G-250 staining. Equal amounts of proteins were separated by SDS-PAGE on a 10% polyacrylamide gel, and the proteins were transferred to a PVDF membrane. After blocking with 0.1% TBST containing 5% nonfat milk at room temperature for 2 h. Primary antibodies (iNOS, ab3525, 1:500 from Abcam, UK; eNOS, ab50010, 1:500 from Abcam, UK; β-actin, Immunoway, YM3028, 1:5000, USA) were incubated with the samples overnight. The membrane was rinsed with 0.1% TBST three times for 10 min each and incubated with secondary antibody (ZB2301, 1:5000, from Santa Cruz Biotechnology, Santa Cruz, CA) at room temperature for 2 h. The color was developed using the ECL kit. Images were acquired using the Fuji Digital Science Imager and analyzed with Gelpro Analyzer (Version 4.0) to measure the integrated optimal density (IOD) values of specific bands.
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