For work involving parasites, chlorpheniramine maleate salt, chlorpromazine hydrochloride, desipramine hydrochloride, promethazine hydrochloride, verapamil hydrochloride and CQ diphosphate (all from Sigma-Aldrich) were dissolved in PBS to a working concentration of 1 mM. LynxTag-CQGREEN (BioLynx Technologies, Singapore; hereafter abbreviated to ‘CQGREEN’) was dissolved in DMSO to the same concentration. All compounds were stored at −20°C and protected from light. For microsome uptake assays, methiothepin mesylate salt, metergoline, loperamide hydrochloride, octoclothepin maleate salt, mibefradil dihydrochloride hydrate, L703,606 oxalate salt hydrate, and chlorprothixene hydrochloride (all from Sigma-Aldrich) were dissolved in DMSO to 10 mM and stored at 4°C. verapamil hydrochloride, adenosine triphosphate (ATP), and CQ diphosphate (all from Sigma-Aldrich) were dissolved in water to 7.5 mM, 50 mM and 0.1 M respectively and stored at −20°C. Tritiated CQ (3H-CQ; from Moravek Biochemicals and Radiochemicals) was diluted in water to 5.32 µM and stored at −20°C; specific activity was 4.7 Ci/mmol.
Chlorprothixene hydrochloride
Manufactured by Merck Group
Chlorprothixene hydrochloride is a chemical compound used in the manufacture of various pharmaceutical products. It is a white or slightly yellowish crystalline powder that is soluble in water and alcohol. The compound is commonly used as a starting material or intermediate in the production of drugs and other medical preparations, but its specific core function and intended use should not be extrapolated upon without further information.
Automatically generated - may contain errors
Lab products found in correlation
Sr 95531 hydrobromide, by Bio-Techne (2 mentions)
Rs cpp, by Bio-Techne (2 mentions)
Nbqx disodium, by Bio-Techne (2 mentions)
Phenylephrine hydrochloride, by Bio-Techne (2 mentions)
Dexmedetomidine hydrochloride, by Bio-Techne (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Prazosin hydrochloride, by Bio-Techne (2 mentions)
Tetrodotoxin citrate, by Alomone (2 mentions)
Chlorpromazine hydrochloride, by Merck Group (1 mentions)
Verapamil hydrochloride, by Merck Group (1 mentions)
Methiothepin mesylate salt, by Merck Group (1 mentions)
Mibefradil dihydrochloride hydrate, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Desipramine hydrochloride, by Merck Group (1 mentions)
Metergoline, by Merck Group (1 mentions)
Promethazine hydrochloride, by Merck Group (1 mentions)
Loperamide hydrochloride, by Merck Group (1 mentions)
Cq diphosphate, by Merck Group (1 mentions)
Bergamo 2, by Thorlabs (1 mentions)
Tl10x 2p, by Thorlabs (1 mentions)
4 protocols using chlorprothixene hydrochloride
1
Antimalarial Compound Preparation and Assay
2
In vivo and in vitro imaging of neuronal activity
3
In vivo and in vitro Imaging Protocols
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For in vivo i.p. injection, chlorprothixene hydrochloride (Sigma-Aldrich, #C1671), prazosin hydrochloride (Tocris, #0623) and dexmedetomidine hydrochloride (Tocris, 2749) were dissolved in DMSO (Sigma-Aldrich, #D2650) at 5 mg/mL, 3 mg/mL and 0.1 mg/mL, respectively. First, 5 minutes of baseline activity was measured using 2P microscopy and then the animals were i.p. injected with equal amounts of DMSO per body weight (1 μL per 1 μg b.w.). Animals were returned to their home cage after injection and then re-mounted under the 2P microscope 20 min after injection for 5 minutes of imaging. For in vitro cortical slice imaging, phenylephrine hydrochloride (Tocris, #2838), tetrodotoxin citrate (Alomone labs #T-550), NBQX disodium (Tocris, #1044), (RS)-CPP (Tocris, #0173) and SR 95531 hydrobromide (Tocris, 1262) were dissolved in ACSF and applied through the superfusing solution.
4
Multimodal Neuronal Activity Imaging
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Rats were anesthetized with isoflurane (4% for induction, 0.5% during recording) and sedated with Chlorprothixene Hydrochloride (intramuscular injection of 300µl of 1 mg/ml solution, Sigma) as was used previously in mice to achieve stable recordings with minimal side effects of isoflurane on neuronal activity [17, 21, 23, 27, 28, 38] . Rats were placed on a heating pad, and were restrained and head-fixed to a custom mount under a two-photon microscope (Bergamo II, Thorlabs). Fluorescence signal was recorded using a 950nm wavelength (50-150 mW, Insight X3, Spectra-Physics) using an 8KHz resonant scanner (Cambridge Technology) and GaAsP photomultiplier tube (Thorlabs PMT2101). We collected light from fields of view (FOVs) with 512x512 pixels at 30 frames per second and with a 16x 0.8NA objective (CFI75 LWD, Nikon) for comparing spontaneous activity between GCaMP6f-and jGCaMP7s-labeled neurons, or a 10x 0.5NA objective lens (TL-10X 2P, Thorlabs) for longitudinal activity recording experiments. The respective FOV size was 200x200 or 500x500 µm 2 .
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