Human neuroblastoma cells (SH-SY5Y cells) were purchased from Procell Life Science & Technology Co., Ltd. SH-SY5Y cells were cultured in MEM/F12 (Gibco) containing 10% foetal bovine serum and 1% penicillin/streptomycin, hereafter referred to marked as SH-SY5Y medium. The cells were maintained at 37 °C in an atmosphere of 5% carbon dioxide and 95% humidity. The induction medium consisted of SH-SY5Y medium supplemented with MPP+(D048, Sigma‒Aldrich) at a concentration of 5 μM. To establish a α-Syn pathological aggregation cell model, we first cultured SH-SY5Y cells on cell culture dishes or coverslips with SH-SY5Y medium for 24 h. Next, we aspirated the original SH-SY5Y medium, washed the cells with PBS, and added an equal volume of induction medium to obtain the induced treatment group. The control group was obtained in a similar way but the SH-SY5Y medium was replaced with fresh SH-SY5Y medium, not induction medium.
Mpp d048
Manufactured by Merck Group
Sourced in United States
The MPP+(D048) is a laboratory equipment product manufactured by Merck Group. It is designed for precise measurements and analysis in various scientific applications. The core function of this product is to provide accurate and reliable data, but a detailed description while maintaining an unbiased and factual approach is not available.
Automatically generated - may contain errors
Lab products found in correlation
Mem f12, by Thermo Fisher Scientific (1 mentions)
Sh sy5y cells, by Thermo Fisher Scientific (1 mentions)
Sh sy5y, by Merck Group (1 mentions)
Sh sy5y cells, by Merck Group (1 mentions)
Sh sy5y cells, by Procell (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Mg132, by Merck Group (1 mentions)
Ab152, by Merck Group (1 mentions)
Anti th, by Merck Group (1 mentions)
Recombinant human hmgb1 1690 hmb, by R&D Systems (1 mentions)
Anti parp, by Santa Cruz Biotechnology (1 mentions)
Anti hmgb1, by Abcam (1 mentions)
Anti gfap, by Abcam (1 mentions)
M0896, by Merck Group (1 mentions)
Anti iba1, by Novus Biologicals (1 mentions)
Anti total jnk, by Cell Signaling Technology (1 mentions)
4 protocols using mpp d048
1
Modulating α-Synuclein Aggregation in Human Neuroblastoma Cells
2
Examining Antigen Presentation in Neuroblastoma Cells
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Human neuroblastoma SH-SY5Y cells were acquired from the Central Laboratory of Nanfang Hospital (Guangzhou, China) and were used to examine the molecular mechanisms of antigen presentation in dopaminergic neurons (Fall and Bennett, 1999; Xicoy et al., 2017). The SH-SY5Y cells were cultured in Dulbecco's Modified Eagle's Medium (Invitrogen, Carlsbad, CA, USA) with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and incubated at 5% CO2 and 37°C. To analyze MHC-I expression in a PD model of SH-SY5Y cells, the cells were treated with 0.1 mM MPP+ (D048: Sigma-Aldrich: St Louis, MO, USA) for 24 hours. Cells in the positive control group were incubated with 100 ng/mL interferon-γ (285-IF-100; R&D Systems, Minneapolis, MN, USA) for 24 hours (Lorenzi et al., 2012, Spel et al., 2015).
3
Microglia-Neuron Interaction in Parkinson's
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Murine microglia-like BV2 cells, SH-SY5Y cells and human embryonic kidney (HEK) 293 T cells were maintained in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Thermo Fisher Scientific) containing 10% fetal bovine serum (FBS; Biological Industries, Beit-Haemek, Israel) in a carbon dioxide incubator at 37 °C. The cells were subcultured every three days. For 1-methyl-4-phenylpyridinium (MPP+; D048, Sigma-Aldrich) treatment, SH-SY5Y cells were treated with 1 mM MPP+ or PBS of equal volume for 24 h. The conditioned media from the SH-SY5Y cells treated with MPP+ (MPP+-CM) or PBS (PBS-CM) were collected and applied to BV2 cells for 24 h.
4
MPTP-Induced Neurodegeneration: Mechanistic Insights
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1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, M0896), 1-methyl-4-phenylpyridinium (MPP + , D048), and MG-132 (M8699) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine 2000 (11668027) was purchased from Thermo-Fisher Scientific (CA, USA). Anti-RAGE (rabbit polyclonal, ab3611) was purchased from Abcam (Cambridge, MA, USA). Anti-phospho JNK (rabbit polyclonal, #9251) and anti-total JNK (rabbit polyclonal, #9252) were purchased from Cell Signaling Technology (Beverly, MA, USA). Anti-GFAP (chicken polyclonal, ab4674) and anti-HMGB1 (rabbit polyclonal, ab18256), were purchased from Abcam (Cambridge, MA, USA). Anti-Iba1 (goat polyclonal, NB100-1028) was purchased from Novus (Littleton, CO, USA). Anti-TH (rabbit polyclonal, AB152) was purchased from Millipore (Temecula, CA, USA). Anti-PARP (rabbit polyclonal, sc-7150), anti-actin (rabbit polyclonal, sc-1616), anti-ubiquitination (mouse monoclonal, sc-8017), and Protein A/G PLUS-agarose beads (sc-2003) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Recombinant human HMGB1 (1690-HMB) was purchased from R&D Systems (Minneapolis, USA). RAGE antagonist/inhibitor (553030) was purchased from Calbiochem (Temecula, CA, USA). JNK inhibitor SP600125 (S5567) was purchased from Sigma-Aldrich.
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