Fitc conjugated annexin 5 annexin 5 fitc propidium iodide apoptosis detection kit

Manufactured by Beyotime

Sourced in China

The FITC-conjugated Annexin V (Annexin V-FITC)/propidium iodide (PI) Apoptosis Detection kit is a tool used to detect and quantify apoptosis in cells. Annexin V-FITC binds to phosphatidylserine exposed on the cell surface during apoptosis, while PI stains the DNA of cells with compromised cell membranes, indicating late apoptosis or necrosis. The kit provides the necessary reagents to perform this analysis.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (2 mentions)

Close spelling variants of this product

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2 protocols using fitc conjugated annexin 5 annexin 5 fitc propidium iodide apoptosis detection kit

Quantifying Apoptosis in Human Retinal Microvascular Endothelial Cells

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FITC-conjugated Annexin V (Annexin V-FITC)/propidium iodide (PI) Apoptosis Detection kit was purchased from Beyotime Biotechnology (Shanghai, China). After treatment and centrifugation at 2000 rpm for 5 min under the condition of 4°C, hRMECs were harvested and added with binding buffer to re-suspend, followed by staining in the dark with Annexin V-FITC and PI for 15 min. A FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, United States) was employed to detect and analyze the apoptosis of hRMECs. The experiments were repeated three times.

Yang K., Liu J., Zhang X., Ren Z., Gao L., Wang Y., Lin W., Ma X., Hao M, & Kuang H. (2020). H3 Relaxin Alleviates Migration, Apoptosis and Pyroptosis Through P2X7R-Mediated Nucleotide Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation in Retinopathy Induced by Hyperglycemia. Frontiers in Pharmacology, 11, 603689.

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Annexin V-FITC/PI Apoptosis Assay in mRECs

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FITC-conjugated Annexin V (Annexin V-FITC)/propidium iodide (PI) Apoptosis Detection kit was purchased from Beyotime Biotechnology (Shanghai, China). After treatment and centrifugation at 2000 rpm for 5 min under the condition of 4 °C, mRECs were harvested and added with binding buffer to re-suspend, followed by staining in the dark with Annexin V-FITC and PI for 15 min. A FACSCalibur flow cytometer (BD Biosciences, Franklin Lakes, NJ, United States) was employed to detect and analyze the apoptosis of mRECs. The experiments were repeated three times.

Kong H., Zhao H., Chen T., Song Y, & Cui Y. (2022). Targeted P2X7/NLRP3 signaling pathway against inflammation, apoptosis, and pyroptosis of retinal endothelial cells in diabetic retinopathy. Cell Death & Disease, 13(4), 336.

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