3 4 hydroxyphenyl propionic acid hppa

Manufactured by Serva Electrophoresis

Sourced in Germany

3-(4-Hydroxyphenyl) propionic acid (HPPA) is a chemical compound used in various laboratory applications. It functions as a reducing agent and is commonly employed in electrophoresis techniques, particularly in the detection and visualization of proteins. HPPA can be utilized to enhance the sensitivity and contrast of protein bands during the electrophoresis process.

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Lab products found in correlation

Glycine, by Serva Electrophoresis (1 mentions) Tween 20, by Roche (1 mentions) Bovine serum albumin (bsa), by Roche (1 mentions)

2 protocols using 3 4 hydroxyphenyl propionic acid hppa

Reagents for Immunoassay Development

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Bovine serum albumin (BSA), Roche universal buffer for ELISA (RUB), phosphate-buffered saline (PBS), and Tween20 (10% (w/v)) were obtained from Roche Diagnostics GmbH (Mannhein, Germany). Rat serum (female, Wistar) was from Charles River Laboratories (Sulzfeld, Germany). Ethanol, 30% H₂O₂, and Tris(hydroxymethyl)aminomethane (Tris/HCl) were ordered from Merck KGaA (Darmstadt, Germany). Glycine and 3-(4-Hydroxyphenyl) propionic acid (HPPA) were obtained from Serva Electrophoresis GmbH (Heidelberg, Germany) and Sigma Aldrich (St. Louis, MO, USA), respectively.

Hoffmann E., Jordan G., Lauer M., Ringler P., Kusznir E.A., Rufer A.C., Huber S., Jochner A., Winter G, & Staack R.F. (2019). Generation, Characterization, and Quantitative Bioanalysis of Drug/Anti-drug Antibody Immune Complexes to Facilitate Dedicated In Vivo Studies. Pharmaceutical Research, 36(9), 129.

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Reagents for Immunoassay Development

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

10x phosphate-buffered saline (PBS, 11666789001), Roche universal buffer for ELISA (RUB), and bovine serum albumin (BSA, 10735094001) were obtained from Roche Diagnostics GmbH (Germany). Rat serum (female, Wistar rats) was obtained from Charles River Laboratories (Germany). Rat plasma (#RATPLLIHP-WH, lot #Rat329266) was obtained from Seralab (UK). 25% HCl (1.00316), ethanol, 30% H₂O₂, and Tris(hydroxymethyl)aminomethane (Tris/HCl) and NaCl (1.06404) were obtained from Merck KGaA (Germany). Histidine was ordered from Sigma (H6034 and 56190, respectively). Glycine and 3-(4Hydroxyphenyl) propionic acid (HPPA) were obtained from Serva Electrophoresis GmbH (Heidelberg, Germany) and Sigma Aldrich (St. Louis, MO, USA), respectively.^{11 (link)}

Opolka-Hoffmann E., Jordan G., Otteneder M., Kieferle R., Lechmann M., Winter G, & Staack R.F. (2021). The impact of immunogenicity on therapeutic antibody pharmacokinetics: A preclinical evaluation of the effect of immune complex formation and antibody effector function on clearance. mAbs, 13(1), 1995929.

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