mIF analysis was performed on TMAs as previously described. Briefly, TMA sections were stained using antibodies against cytokeratins (clone C‐11, dilution 1:250; Abcam), PD‐1 (clone NAT105, dilution 1:100; Abcam), PD‐L1 (clone E1L3N, dilution 1:100; Cell Signaling Technologies), CD8 (clone D8A8Y, dilution 1:200; Cell Signaling Technologies), CD4 (clone EP204, dilution 1:100; Cell Signaling Technologies), CD68 (clone D4B9C, dilution 1:400; Cell Signaling Technologies) and CTLA4 (clone CAL49, dilution 1:200; Abcam). All antibodies were linked with one of the fluorophores from the Opal 7 IHC kit, (NEL797001KT; Akoya Biosciences). Sections were scanned by a tissue imaging system (Aperio Versa 8; Leica). Imaging analysis was performed with quantitative image analysis software (Halo; Indica Labs).
Ctla4 clone cal49
Manufactured by Abcam
Sourced in United States
CTLA4 (clone CAL49) is a monoclonal antibody that binds to the human CTLA4 (cytotoxic T-lymphocyte-associated protein 4) antigen. CTLA4 is a T cell surface receptor that plays an important role in the regulation of T cell activation and immune response.
Automatically generated - may contain errors
Lab products found in correlation
Pd l1 clone e1l3n, by Cell Signaling Technology (2 mentions)
Clone c 11, by Abcam (2 mentions)
Cd4 clone ep204, by Cell Signaling Technology (2 mentions)
Cd68 clone d4b9c, by Cell Signaling Technology (2 mentions)
Cd8 clone d8a8y, by Cell Signaling Technology (2 mentions)
Pd 1 clone nat105, by Abcam (2 mentions)
Aperio versa 8, by Leica (1 mentions)
2 protocols using ctla4 clone cal49
1
Multiplex Immunofluorescence Analysis of TMAs
2
Multiplexed Immunofluorescence Analysis of Tumor Microenvironment
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mIF analysis was performed on TMAs. TMA sections were stained using antibodies against CD4 (clone EP204, dilution 1:100, Cell Signaling Technologies, Boston, USA), CD8 (clone D8A8Y, dilution 1:200, Cell Signaling Technologies), CD68 (clone D4B9C, dilution 1:400, Cell Signaling Technologies), programmed cell death 1 (PD-1) (clone NAT105, dilution 1:100, Abcam), PD-L1 (clone E1L3N, dilution 1:100, Cell Signaling Technologies), CTLA-4 (clone CAL49, dilution 1:200, Abcam), and cytokeratins (clone C-11, dilution 1:250, Abcam). All antibodies were linked with one of the fluorophores from the Opal 7 IHC kit, (Cat No. NEL797001KT; Akoya Biosciences, MA, USA). Sections were scanned by Aperio Versa 8 tissue imaging system (Leica, Hesse, Germany). Imaging analysis was performed with a quantitative image analysis software (Halo, Indica Labs, New Mexico, USA). X-tile was used to determine the cut-off points for survival analysis.
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