Total cellular proteins were prepared as previously described [19 (link)]. For western blot analysis, 20 mg of cellular protein was subjected to sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) on a 4–15% gradient gel (Bio-Rad). The blot was transferred onto a polyvinylidene difluoride membrane using the iBlot 2 (Life Technologies), followed by blocking with Tris-buffered saline (Takara Bio, Shiga, Japan) containing 0.2% dry fat-free milk (Cell Signaling). Primary antibody reaction, horseradish-peroxidase-conjugated secondary antibody (NA934V, GE Healthcare UK Ltd., Buckinghamshire, UK) reaction, and washing steps have been previously described [30 (link)]. Bands were visualized using Amersham ECL western blotting detection reagent (GE Healthcare UK Ltd) and the ChemiDoc XRS Plus ImageLab system (Bio-Rad).
Horseradish peroxidase conjugated secondary antibody na934v
Manufactured by GE Healthcare
Sourced in United Kingdom
The Horseradish-peroxidase-conjugated secondary antibody (NA934V) is a lab equipment product designed for use in immunoassay techniques. It functions as a detection reagent that binds to primary antibodies, enabling visualization and quantification of target analytes in samples.
Automatically generated - may contain errors
Lab products found in correlation
Dry fat free milk, by Cell Signaling Technology (2 mentions)
Chemidoc xrs plus imagelab system, by Bio-Rad (2 mentions)
Iblot2, by Thermo Fisher Scientific (2 mentions)
Gradient gel, by Bio-Rad (2 mentions)
Amersham ecl western blotting detection reagent, by GE Healthcare (2 mentions)
Tris buffered saline (tbs), by Takara Bio (2 mentions)
Amersham ecl western blotting, by Cytiva (2 mentions)
2 protocols using horseradish peroxidase conjugated secondary antibody na934v
1
Western Blot Analysis of Cellular Proteins
2
Western Blot Analysis of Total Cellular Proteins
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Total cellular proteins were prepared as previously described [19] . For western blot analysis, 20 mg of cellular protein was subjected to sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 4-15% gradient gel (Bio-Rad). The blot was transferred onto a polyvinylidene difluoride membrane using the iBlot 2 (Life Technologies), followed by blocking with Tris-buffered saline (Takara Bio, Shiga, Japan) containing 0.2% dry fat-free milk (Cell Signaling). Primary antibody reaction, horseradish-peroxidase-conjugated secondary antibody (NA934V, GE Healthcare UK Ltd., Buckinghamshire, UK) reaction, and washing steps have been previously described [29] . Bands were visualized using Amersham ECL western blotting detection reagent (GE Healthcare UK Ltd) and the ChemiDoc XRS Plus ImageLab system (Bio-Rad).
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