Alkaline phosphatase alp kit

The animal experiment was approved by the Experimental Animal Care Ethics Committee of Northwest A&F University. A total of 320 ICR mice (male, 9 weeks old) with an average weight of 33 ± 2 g, SPF grade, were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) (Approval Number: SCXK (Beijing) 2012-0001). Before the experiment, all experimental animals were bred adaptively in the animal room for one week. The conditions in the animal room were controlled as follows: the humidity was set to 55% ± 5% and the temperature was set to 25 ± 2 ℃, a 12-h circadian rhythm was ensured, and the experimental animals could drink water and eat freely. After all the mice had adapted to the environment, the experiment was started.
The aspartate aminotransferase (AST) kit, the alanine aminotransferase (ALT) kit, the total bilirubin (TBIL) kit, the alkaline phosphatase (ALP) kit, the total triglyceride (TC) kit, the total cholesterol (TG) kit, the total antioxidant capacity (T-AOC) kit, the catalase (CAT) kit, the glutathione (GSH) kit, the superoxide dismutase (SOD) kit, and the malonaldehyde (MDA) kit were purchased from Nanjing Jiancheng Bioengineering Institute (China).

FA (purity ≥ 99%) was purchased from Solarbio Science and Technology Co., Ltd. (Beijing, China). Primers (Table 1) used for qRT-PCR were ordered from Sangon Biotechnology Ltd. (Shanghai, China). Commercial detection kits for alanine transaminase (ALT), aspartate transaminase (AST), total cholesterol (TC), triglycerides (TG) were obtained from Changchun Huili Biotech Co., Ltd. (Jilin, China). Mouse Insulin Elisa Kit (ED-20778) was obtained from Lunchangshuo Biotech Co., Ltd. Alkaline phosphatase (ALP) kit, γ-glutamytransferase (GGT) kit and total BA kit (E003-2-1) were obtained from Nanjing Jiancheng Bioengineering Institute. Other chemicals and reagents were purchased from Sigma-Aldrich (Germany) unless specifically stated.