Buffered 10 formalin

Manufactured by Thermo Fisher Scientific

Sourced in Canada

Buffered 10% formalin is a fixative solution used in laboratory settings. It is a mixture of 10% formaldehyde and a buffer, typically phosphate or citrate. This solution is designed to preserve biological samples by crosslinking proteins, thereby maintaining the structural integrity of cells and tissues.

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Lab products found in correlation

Phenol red free matrigel, by Corning (1 mentions) Hepes buffered saline, by Thermo Fisher Scientific (1 mentions) Anti c met, by Abcam (1 mentions)

Spelling variants of this product

Formalin (259 citations) Buffered formalin (153 citations) PROTOCOL™ 10% Buffered Formalin (4 citations) Buffered 10% formalin solution (2 citations) Shandon Formal-Fixx 10% neutral buffered formalin (2 citations)

2 protocols using buffered 10 formalin

Adhesion of B. burgdorferi to HUVEC

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As described^{12 (link), 65 (link)}, HUVEC were grown to confluence in 4-well chamber slides (Nalgene Nunc International, Rochester, NY, USA) coated with 500 μg/mL phenol red-free matrigel (Corning, Tewksbury, MA, USA). GFP-expressing B. burgdorferi (1.4 × 10⁷) were resuspended in a 3:1 mixture of BSK-II:EGM-2 and added to duplicate wells containing HUVEC. For competitive inhibition experiments, bacteria or HUVEC were pre-incubated with 8 μg of plasma fibronectin (pFn) (Sigma, Oakville, Canada) for 1 hour. Chamber slides were incubated for 12 h, washed with HEPES-buffered saline, and fixed in buffered 10% formalin (Fisher Scientific, Ottawa, Canada). B. burgdorferi adhered to HUVEC were counted in 10 fields of view/biological replicate using a Nikon 80i fluorescence microscope (Meridian Instrument Company, Kent, WA, USA).

Kao W.C., Pětrošová H., Ebady R., Lithgow K.V., Rojas P., Zhang Y., Kim Y.E., Kim Y.R., Odisho T., Gupta N., Moter A., Cameron C.E, & Moriarty T.J. (2017). Identification of Tp0751 (Pallilysin) as a Treponema pallidum Vascular Adhesin by Heterologous Expression in the Lyme disease Spirochete. Scientific Reports, 7, 1538.

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Immunohistochemical Analysis of Bone Biopsy

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Bone biopsy cores were immediately placed in buffered 10% formalin (Fisher Scientific, Waltham, MA) overnight at 4°C, rinsed with PBS then transferred to Immunocal (Decal Chemical Corporation, Tallman, NY) to decalcify for 1 to 2 hours. Samples were paraffin embedded, sectioned and stained with hematoxylin and eosin for immunohistochemistry. Antibodies used were: anti-cytokeratin CAM5.2 (BD349205; Becton-Dickinson. Franklin Lakes, NJ); anti-CMET (Cat# 51067; Abcam, Cambridge, MA), anti-pCMET (Cat# 44–888G; Invitrogen, Carlsbad, CA) and anti-VEGFR2/Flk-1 (SC-504; Santa Cruz Biotechnologies, Santa Cruz, CA) at 1:20, 1:200, 1:50, and 1:150 respectively. Antigen retrieval was performed with Diva Decloaker (DV2004, Biocare Medical, Concord, CA) for CMET and pCMET, and with Reveal (RV1000, Biocare Medical) for VEGFR2. Antibodies were optimized by titration using prostate cancer tissues. Results are presented as the expression index (EI) which is the product of the staining intensity x the percent positive cells.

Smith D.C., Daignault-Newton S., Grivas P., Reichert Z.R., Hussain M., Cooney K.A., Caram M., Alva A., Jacobson J., Yablon C., Mehra R., Escara-Wilke J., Shelley G, & Keller E.T. (2020). Efficacy and Effect of Cabozantinib on Bone Metastases in Treatment-Naïve Castration-Resistant Prostate Cancer. Clinical genitourinary cancer, 18(4), 332-339.e2.

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