Three genes (>2-fold change) that had antithrombotic function were verified by qPCR technique. PrimeScript RT Master Mix (Takara, Beijing, China) was used to obtain the cDNA. qPCR was performed according to the instructions of SYBR Premix Ex Taq II quantitative augmentation reaction system (Takara). The reaction was incubated in an ABI PRISM 7300 Fast Real-Time PCR System at the condition of SYBR Premix Ex Taq II quantitative augmentation reaction system (Takara). The primer sequences used for Real-Time RT-PCR were shown in Table 1, and all primers were synthesized by Sangon Biotech Company (Shanghai, China).
Zhang X., Zhang C., Sai J., Li F., Liu J., Li Y, & Wang F. (2015). Xueshuan Xinmaining Tablet Treats Blood Stasis through Regulating the Expression of F13a1, Car1, and Tbxa2r. Evidence-based Complementary and Alternative Medicine : eCAM, 2015, 704390.
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