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Anti mouse integrin b7 bv421

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Anti-mouse Integrin B7 BV421 is a fluorochrome-conjugated antibody that binds to the mouse Integrin β7 protein. Integrin β7 is a cell surface receptor involved in cell-cell and cell-extracellular matrix interactions.

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Lab products found in correlation

Anti mouse cd19 percpcy5, by BioLegend (2 mentions) Anti mouse cd3 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti mouse iga pe, by Thermo Fisher Scientific (2 mentions) Anti mouse cd103 apc, by Thermo Fisher Scientific (2 mentions) Ls column, by Miltenyi Biotec (2 mentions) Facsdiva software, by BD (2 mentions) Facsaria, by BD (2 mentions) Cd3ε microbead kit, by Miltenyi Biotec (1 mentions)

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Mouse anti (2 citations)

2 protocols using anti mouse integrin b7 bv421

1

Isolation and Characterization of Intestinal B Cells

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Single-cells suspension of LPMC were depleted of CD3+ cells using CD3ɛ MicroBead Kit (130-094-973, Miltenyi Biotec) and LS columns (130-042-401, Miltenyi Biotec) and stained with anti-mouse CD19 PerCpCy5.5 (115534, Biolegend, San Diego, CA), anti-mouse CD3 APC-eFluor 780 (47-0032-82, Invitrogen), anti-mouse IgA PE (12-4204-83, Invitrogen), anti-mouse CD103 APC (17-1031-82, Invitrogen) and anti-mouse Integrin B7 BV421 (564283, BD Bioscience, La Jolla, CA) and sorted on a fluorescence-activated cell sorter FACSAria (BD Biosciences) using FACSDiva software (BD Biosciences).
Guzman M., Lundborg L.R., Yeasmin S., Tyler C.J., Zgajnar N.R., Taupin V., Dobaczewska K., Mikulski Z., Bamias G, & Rivera-Nieves J. (2021). An integrin αEβ7-dependent mechanism of IgA transcytosis requires direct plasma cell contact with intestinal epithelium. Mucosal Immunology, 14(6), 1347-1357.
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2

Isolation and Sorting of Intestinal Lymphocytes

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Single-cells suspension of LPMC were depleted of CD3+ cells using CD3ε MicroBead Kit (130-094-973, Miltenyi Biotec) and LS columns (130-042-401, Miltenyi Biotec) and stained with anti-mouse CD19 PerCpCy5.5 (115534, Biolegend, San Diego, CA), anti-mouse CD3 APC-eFluor 780 (47-0032-82, Invitrogen), anti-mouse IgA PE (12-4204-83, Invitrogen), anti-mouse CD103 APC (17-1031-82, Invitrogen) and anti-mouse Integrin B7 BV421 (564283, BD Bioscience, La Jolla, CA) and sorted on a fluorescence‐activated cell sorter FACSAria (BD Biosciences) using FACSDiva software (BD Biosciences).
Guzman M., Lundborg L.R., Yeasmin S., Tyler C.J., Zgajnar N.R., Taupin V., Dobaczewska K., Mikulski Z., Bamias G, & Rivera-Nieves J. (2021). An integrin αEβ7-dependent mechanism of IgA transcytosis requires direct plasma cell contact with intestinal epithelium. Mucosal Immunology, 14(6), 1347-1357.
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