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Ctgf or ccn3

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CTGF or CCN3 is a protein that belongs to the CCN family of extracellular matrix-associated proteins. It plays a role in various cellular processes, including cell growth, differentiation, and adhesion. The core function of CTGF is to act as a regulator of cellular activities.

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Lab products found in correlation

Tracp alp assay kit, by Takara Bio (2 mentions) Osteoassay bone plates, by Lonza (2 mentions) Microvue bone helical peptide eia assay, by Quidel (2 mentions)

2 protocols using ctgf or ccn3

1

Quantification of Osteoclast Differentiation and Activity

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Human osteoclasts were obtained from Lonza and differentiated in vitro according to vendor’s instructions. Conditioned media collected from the KO cells or cells transfected with indicated LNAs/plasmids after 72 hours were added to osteoclast cells at day 3 of differentiation in a 6-well plate. In rescue experiments, recombinant CTGF or CCN3 (PeproTech) was supplemented in conditioned media as indicated. Cells were TRAP stained on day 7 using Acid Phosphatase, Leukocyte (TRAP) kit (Sigma-Aldrich), and TRAP+-multinucleated cells were quantified as mature osteoclasts. Quantitative TRAP assay was performed using TRACP & ALP assay kit (Takara). For osteoclast resorption function analyses, bone marrow osteoclast differentiation was conducted in OsteoAssay bone plates (Lonza) and osteoclast activity was determined by quantifying the type I collagen helical peptide α1 (I) 620–633 released from bone into culture medium using MicroVue Bone Helical Peptide EIA assay (Quidel).
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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2

Quantification of Osteoclast Differentiation and Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human osteoclasts were obtained from Lonza and differentiated in vitro according to vendor’s instructions. Conditioned media collected from the KO cells or cells transfected with indicated LNAs/plasmids after 72 hours were added to osteoclast cells at day 3 of differentiation in a 6-well plate. In rescue experiments, recombinant CTGF or CCN3 (PeproTech) was supplemented in conditioned media as indicated. Cells were TRAP stained on day 7 using Acid Phosphatase, Leukocyte (TRAP) kit (Sigma-Aldrich), and TRAP+-multinucleated cells were quantified as mature osteoclasts. Quantitative TRAP assay was performed using TRACP & ALP assay kit (Takara). For osteoclast resorption function analyses, bone marrow osteoclast differentiation was conducted in OsteoAssay bone plates (Lonza) and osteoclast activity was determined by quantifying the type I collagen helical peptide α1 (I) 620–633 released from bone into culture medium using MicroVue Bone Helical Peptide EIA assay (Quidel).
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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