Rt sybr green qpcr master mixes

Manufactured by Qiagen

RT² SYBR Green qPCR Master Mixes are ready-to-use solutions for quantitative real-time PCR (qPCR) analysis. They contain SYBR Green I dye, which binds to double-stranded DNA and emits fluorescence, enabling real-time detection and quantification of PCR products.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Ez magna chip g chromatin immunoprecipitation kit, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Lightcycler 480 real time pcr system, by Roche (1 mentions) Qscripttm cdna supermix, by Quantabio (1 mentions) C1000 touchtm thermal cycler, by Bio-Rad (1 mentions)

2 protocols using rt sybr green qpcr master mixes

ChIP-PCR Assay Protocol

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Chromatin immunoprecipitation (ChIP) assays were performed according to the protocol from Millipore (EZ-Magna ChIP G Chromatin Immunoprecipitation Kit). DNA was eluted with 100 ul elution buffer, and 4 ul was used per Q-PCR analysis. Quantitative PCR was performed using RT² SYBR Green qPCR Master Mixes (Qiagen) in a LightCycler 480 Real-Time PCR System (Roche). The antibodies used in the ChIP-PCR analysis were anti-Flag (Sigma, F1804); anti-trimethyl-Histone H3K4 (07-473), anti-trimethyl-Histone H3K9 (07-442) and anti-mouse IgG (12-371) were from Merck Millipore. The primers used in this study are shown in Supplementary Table S5.

Su P.H., Lai H.C., Huang R.L., Chen L.Y., Wang Y.C., Wu T.I., Chan M.W., Liao C.C., Chen C., Lin W.Y, & Chang C.C. (2019). Paired Box-1 (PAX1) Activates Multiple Phosphatases and Inhibits Kinase Cascades in Cervical Cancer. Scientific Reports, 9, 9195.

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Testis RNA Extraction and qRT-PCR

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Total RNA was extracted from testis by TRIzol (ThermoFisher), and the reverse transcription was performed using a qScript TM cDNA SuperMix (Quantabio, 95048), according to the manufacturer's instructions. For qRT-PCR, the specificity of PCRs was verified by a single peak according to melt curves. The primer sequences are listed in Table S1. qRT-PCR was performed with a C1000 Touch TM Thermal Cycler (Bio-Rad) amplification system using RT² SYBR ® Green qPCR Mastermixes (Qiagen). The relative levels of transcripts were calculated using the 2 -ΔΔCT method and the Rabl2 expression levels were normalized to Gapdh.

Ding X., Fragoza R., Singh P., Zhang S., Yu H., & Schimenti J.C. (2020). Variants in RABL2A causing male infertility and ciliopathy.

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