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Fitc conjugated anti igm

Manufactured by Thermo Fisher Scientific
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FITC-conjugated anti-IgM is a laboratory reagent used for the detection and identification of immunoglobulin M (IgM) in biological samples. The product consists of polyclonal antibodies specific to human IgM that are conjugated to the fluorescent dye fluorescein isothiocyanate (FITC).

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Lab products found in correlation

Pe conjugated anti iga, by Southern Biotech (3 mentions) Facscanto 2, by BD (2 mentions) Facscalibur, by BD (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FITC-conjugated anti-murine IgM antibody FITC-conjugated goat-anti-mouse IgM FITC-conjugated goat anti-human IgM or IgG antibody Anti-IgM-FITC IgM-FITC

3 protocols using fitc conjugated anti igm

1

IgA Class Switching in CH12 Cells

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CH12 cells were cultured and stimulated with 250ng/mL CD40L, 10ng/mL IL4 and 1ng/mL TGFβ (CIT) to induce class switching to IgA. For siRNA-based knockdown experiments, transfection of siRNA oligonucleotide (Invitrogen) into CH12 cells was done using electroporation kit (Lonza). The cells were subsequently cultured for 24 hours before the addition of CIT and incubated further for an additional 24 hours. For FACS analysis, the cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech). All analyses were performed on FACS Calibur (Becton Dickinson).
Gupta R., Somyajit K., Narita T., Maskey E., Stanlie A., Kremer M., Typas D., Lammers M., Mailand N., Nussenzweig A., Lukas J, & Choudhary C. (2018). DNA Repair Network Analysis Reveals Shieldin as a Key Regulator of NHEJ and PARP Inhibitor Sensitivity. Cell, 173(4), 972-988.e23.
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2

Mouse B Lymphocyte Class Switching

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The mouse B lymphocyte cell line CH12-F3 cells was cultured and stimulated with 250 ng/ml CD40L, 10 ng/ml IL4 and 1 ng/ml TGFβ (CIT) to induce immunoglobulin class switching from IgM to IgA. For FACS analysis of CSR efficiency, live CH12-F3 cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech) antibodies. All analyses were performed on FACS CantoII (BD Biosciences).
Shinoda K., Zong D., Callen E., Wu W., Dumitrache L.C., Belinky F., Chari R., Wong N., Ishikawa M., Stanlie A., Multhaupt-Buell T., Sharma N., Ozelius L., Ehrlich M., McKinnon P.J, & Nussenzweig A. (2021). The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice. Molecular cell, 81(12), 2611-2624.e10.
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3

Induced IgM to IgA Class Switching

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The mouse B lymphocyte cell line CH12-F3 cells was cultured and stimulated with 250 ng/ml CD40L, 10 ng/ml IL4 and 1 ng/ml TGFb (CIT) to induce immunoglobulin class switching from IgM to IgA. For FACS analysis of CSR efficiency, live CH12 cells were labeled with FITC-conjugated anti-IgM (ThermoFisher Scientific) and PE-conjugated anti-IgA (Southern Biotech) antibodies. All analyses were performed on FACS CantoII (BD Biosciences).
Shinoda K., Zong D., Callén E., Wu W., Dumitrache L.C., Belinky F., Wong N., Ishikawa M., Stanlie A., Ehrlich M.E., McKinnon P.J., & Nussenzweig A. (2020). The Dystonia Gene THAP1 Controls DNA Double Strand Break Repair Choice.
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