For immunohistochemistry, some sections were incubated in goat normal serum (in order to block the nonspecific site), and subsequently with anti-caspase 3 rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam, Cambridge, USA), anti-COX 2 rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam, Cambridge, USA), and anti-PARP rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam, Cambridge, USA) overnight at 4 °C. The sections were washed with PBS and then incubated with secondary antibody conjugated with horseradish peroxidase (goat anti-rabbit IgG, Abcam, Cambridge, USA) for 2 hours and detected by diaminobenzidine tetrahydrochloride for 5 minutes. Afterward, they were dehydrated and mounted. For negative controls, primary antibodies were omitted. For quantitative analysis, immunohistochemical photographs (n=5 photos from each sample were collected from all the mice in each experimental group) were assessed by densitometry using MacBiophotonics ImageJ 1.41a software on an ASUS personal computer. The data are expressed as a percentage of the total tissue area.
Anti cox 2 rabbit polyclonal antibody
Manufactured by Abcam
Sourced in United States
Anti-COX 2 rabbit polyclonal antibody is a laboratory reagent used to detect the presence and quantity of COX-2 protein in samples. This antibody is produced in rabbits and recognizes the COX-2 protein, which plays a role in inflammatory processes.
Automatically generated - may contain errors
3 protocols using anti cox 2 rabbit polyclonal antibody
1
Immunohistochemical Analysis of Apoptosis Markers
2
Immunohistochemical Analysis of Spinal Cord and DRG
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For immunohistochemistry, some sections were incubated with anti-caspase 3 rabbit polyclonal antibody (1:200 in PBS, v/v, Abcam), anti-COX 2 rabbit polyclonal antibody (1:200 in PBS, v/v, Abcam), and anti-S100ß rabbit polyclonal antibody (1:500 in PBS, v/v, Abcam) overnight at 4 °C. Sections were washed with PBS and incubated with secondary antibody conjugated with horseradish peroxidase (goat anti-rabbit IgG, Abcam) for 2 h. For quantitative analysis, immunohistochemical photographs (n = five photos from each five-micrometer serial transverse sections of ipsilateral spinal cord segments of the sciatic nerve and related dorsal root ganglions, the thickness of between sampled sections was 48 μm for spinal cord and 36 μm for DRG) from all rats in each experimental group were assessed by densitometry using ImageJ software. Data are expressed as a percentage of total tissue area.
3
Immunohistochemical Analysis of Apoptosis and Inflammation Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For immunohistochemistry, sections were incubated in normal serum (in order to block non-specific site), and then with anti-caspase 3 rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam), anti-COX 2 rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam) and anti-PARP rabbit polyclonal antibody (1:100 in PBS, v/v, Abcam) overnight at 4 °C. Sections were washed with PBS and then incubated with secondary antibody conjugated with horseradish peroxidase (goat anti-rabbit IgG, Abcam) for 2 h and detected by diaminobenzidine tetrahydrochloride for 5 min. After wards, they were dehydrated and mounted. For negative controls, primary antibodies were omitted. For quantitative analysis at percent of total tissue area, immunohistochemical photographs (n = 5 photos from each samples collected from all mice in each experimental group) were assessed by densitometry using MacBiophotonics ImageJ 1.41a software on an ASUS personal computer. Data are expressed as a percentage of total tissue area.
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