The cell lysates were both centrifuged at 13,000 rpm (at 4°C for 5 min). After that 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) were used to segregate them and then transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Bedford, MA, USA). The filters were blocked with blocking buffer (Sangon Biotech) for 45 min. Membranes were incubated with primary antibodies anti-LRH1 (Abcam, ab223211), anti-E-cadherin (Abcam, ab1416), anti-N-cadherin (Abcam, ab18203), anti-Vimentin (Abcam, ab92547), anti-β-catenin (Cell Signaling Technology, D10A8)) at 4°C overnight and with secondary antibodies at 1:5,000 dilution at room temperature for 2 h, followed by incubating with β-actin (Abcam, ab179467) at room temperature for 1.5 h.
Ab223211
Manufactured by Abcam
Ab223211 is a lab equipment product offered by Abcam. It is a device designed for use in scientific research and laboratory settings. The core function of this product is to provide a specific capability or tool for conducting experiments or analyses, but a detailed description of its intended use or technical specifications is not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab223211
1
Western Blot Analysis of EMT Markers
2
Western Blot Analysis of LRH1 Protein
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The cell lysates were both centrifuged at 13000 rpm (at 4℃ for 5 minutes). After that 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) were used to segregate them, and then transferred onto polyvinylidene di uoride (PVDF) membranes (Millipore, Bedford, MA, USA).The lters were blocked with blocking buffer (Sangon Biotech) for 45 min.Membranes were incubated with Primary antibodies against LRH1 (Abcam, ab223211) at 4℃ overnight, and with secondary antibodies at 1:5000 dilution at room temperature for 2 h, following by incubating with β-actin (Abcam, ab179467) atroom temperature for 1.5 h.
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