C009 3 1

Manufactured by Nanjing Jiancheng

Sourced in China

The C009-3-1 is a laboratory equipment product. It is designed to perform a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach. Further information is not available.

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Lab products found in correlation

C010 3 1, by Nanjing Jiancheng (4 mentions) Au480, by Beckman Coulter (2 mentions) A110 1 1, by Nanjing Jiancheng (2 mentions) A111 1 1, by Nanjing Jiancheng (2 mentions) A112 1 1, by Nanjing Jiancheng (2 mentions) A113 1 1, by Nanjing Jiancheng (2 mentions) A042 2 1, by Nanjing Jiancheng (1 mentions)

4 protocols using c009 3 1

Serum Liver Enzyme and Lipoperoxide Analysis

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Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured by the enzymatic kinetic method (cat. nos. C009-3 -1 and C010-3-1; Nanjing Jiancheng Bioengineering Institute) using an automatic biochemical analyser (UA2700; Olympus Corporation) according to the manufacturer's protocol.
Liver lipoperoxide levels were estimated by measuring malondialdehyde (MDA) levels using the OxiSelect™ thiobarbituric acid-reactive substances (TBARS) Assay kit (MDA Quantitation) (cat. no. DTBA-100; BioAssay Systems) according to the manufacturer's protocol.

Kong L., An X., Hu L., Zhang S., Liu L., Zhao S., Wang R, & Nan Y. (2022). Resveratrol ameliorates nutritional steatohepatitis through the mmu-miR-599/PXR pathway. International Journal of Molecular Medicine, 49(4), 47.

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Metabolic Profile Analysis in Mice

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After the end of the drug regimen, the mice were deprived of water and food for 12 h, and the mice were anesthetized with sodium pentobarbital (60 mg/kg) by intraperitoneal injection (i.p.). Then, blood was collected from the orbit by using vacuum anticoagulant tubes. The blood samples were placed in vacuum anticoagulant tubes for 10 min and centrifuged at 1500 g/min at room temperature for 20 min to collect serum. The levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), free fatty acids (FFA), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were measured by biochemical assay according to the corresponding kit’s instructions (cat No. C009-3-1, C010-3-1, A110-1-1, A111-1-1, A042-2-1, A112-1-1, A113-1-1; Nanjing Jiancheng Bioengineering Institute, Nanjing, China) by using an automatic biochemical analyzer (AU480, Beckman Coulter, Miami, FL, USA), respectively. The levels of fasting blood glucose (FBG) and fasting insulin (FINS) were quantified by kits assay according to the corresponding kit’s instructions (cat No. YC0713, YS02720B; Shanghai YaJi Biotechnology Co., Ltd., Shanghai, China). The degree of IR was evaluated by using a homeostasis model assessment of IR index (HOMA-IR). The HOMA-IR was calculated as FBG (mmol/L) × FINS (μU/mL)/22.5.

Li L., Yang L., Yang F., Zhao X.L., Xue S, & Gong F.H. (2021). Ginkgo biloba Extract 50 (GBE50) Ameliorates Insulin Resistance, Hepatic Steatosis and Liver Injury in High Fat Diet-Fed Mice. Journal of Inflammation Research, 14, 1959-1971.

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Blood Collection and Biochemical Analysis

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After the 8-week experiment concluded, animals that had fasted overnight were sedated with 100% isoflurane (2 mg/kg body weight or equivalent to 2% inhaled concentration) and dissected. Using a dry Eppendorf tube, blood was collected via cardiac puncture, allowed to coagulate for 30 min at room temperature, and then centrifuged for 10 to 15 min at 3000 rpm.
Nonhemolyzed serum samples were promptly collected and stored at −20 °C for the evaluation of various parameters, including total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), fasting blood glucose (FBG), insulin, homeostatic model assessment for insulin resistance (HOMA-IR), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels. The levels of TC, TG, HDL-C, LDL-C, AST, and ALT were determined using biochemical assays following the instructions provided with the corresponding kits (Cat. No. A110-1-1, A111-1-1, A112-1-1, A113-1-1, C009-3-1, and C010-3-1, respectively, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Additionally, FBG levels were measured using a kit according to the manufacturer’s instructions (Cat. No. YC0713; Shanghai YaJi Biotechnology Co., Ltd., Shanghai, China). These assays were conducted by using an automatic biochemical analyzer (AU480 from Beckman Coulter, Miami, FL, USA).

Nofal A.E., AboShabaan H.S., Fadda W.A., Ereba R.E., Elsharkawy S.M, & Hathout H.M. (2024). L-carnitine and Ginkgo biloba Supplementation In Vivo Ameliorates HCD-Induced Steatohepatitis and Dyslipidemia by Regulating Hepatic Metabolism. Cells, 13(9), 732.

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Measuring ALT and AST in Culture Supernatant

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After indicated treatment, cell-free culture supernatant was harvested for measuring ALT and AST. The activities of ALT and AST in medium were measured with commercial assay kits from the Jiancheng Bioengineering Institute (ALT, C009-3-1 and AST, C010-3-1). Assays were performed according to the manufacturers' instruction. For ALT, the coefficient of variation of intra-assay, interassay, and the limit of quantification was 2.2%, 5.45%, and 0.01 U/L, respectively. For AST, the coefficient of variation of intra-assay, interassay, and the limit of quantification was 3.0%, 6.95%, and 0.01 U/L, respectively.

Gao W., Fang Z., Lei L., Ju L., Jin B., Loor J.J., Liang Y., Shi Z., Shen T., Yu H., Chen M., Ouyang H., Song Y., Wang Z., Liu G., Li X, & Du X. (2021). Propionate alleviates palmitic acid-induced endoplasmic reticulum stress by enhancing autophagy in calf hepatic cells. Journal of dairy science, 104(8).

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