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Adenosine diphosphate (adp)

Manufactured by Bio-Techne
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The ADP is a laboratory instrument designed for the analysis and quantification of adenosine diphosphate (ADP) molecules. It serves as a tool for researchers to measure ADP levels in various biological samples, such as cell lysates or tissue extracts.

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Lab products found in correlation

Rolipram, by Bio-Techne (2 mentions) 3 isobutyl 1 methyl xanthine (ibmx), by Bio-Techne (2 mentions) Ar c66096, by Bio-Techne (2 mentions) Ar c 69931, by Bio-Techne (2 mentions) Lps from e coli 0111 b4, by Merck Group (2 mentions) Sq22536, by Bio-Techne (1 mentions) Win64338, by Bio-Techne (1 mentions) Gsk 7975a, by GlaxoSmithKline (1 mentions) Mrs2179, by Bio-Techne (1 mentions) Mesadp, by Bio-Techne (1 mentions) Mrs2365, by Bio-Techne (1 mentions) Mrs2211, by Bio-Techne (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) Fluo 4 am, by Thermo Fisher Scientific (1 mentions)

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Adenosine (7 citations)

3 protocols using adenosine diphosphate (adp)

1

Pretreatment and LPS Stimulation Assay

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Cells were pretreated for 1 h with drug treatment. ITI-214 was synthesized at Intra-Cellular Therapies, Inc. (Li et al., 2016 (link)). Other drugs were purchased from Sigma-Aldrich (ADP, IBMX, rolipram) or Tocris (AR-C 66096, and AR-C 69931). Where indicated, following pretreatment, LPS (from E. coli 0111:B4, Millipore-Sigma) was added to the media at 50 ng/mL for 4 h. At 5 h, the cells or media were harvested and processed as indicated below.
O'Brien J.J., O'Callaghan J.P., Miller D.B., Chalgeri S., Wennogle L.P., Davis R.E., Snyder G.L, & Hendrick J.P. (2019). Inhibition of calcium-calmodulin-dependent phosphodiesterase (PDE1) suppresses inflammatory responses. Molecular and cellular neurosciences, 102, 103449.
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2

Pharmacological Modulation of Immune Cells

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BK, S-BK, WIN64338, MeSADP, MRS 2365, MRS 2179, MRS 2211, ADP, SQ 22536 were purchased from Tocris Biosciences (Bristol, UK). GSK7975A was a gift from GlaxoSmithKline (Stevenage, UK). Fluo-4 AM and Hoechst 33342 were purchased from Invitrogen (Life Technologies, Carlsbad, CA, USA). Mouse F4/80 monoclonal rat Antibody (CI-A3-1) [Alexa Fluor® 647] and mouse CD11b/Integrin alpha M Alexa Fluor® 647-conjugated monoclonal rat antibodies were obtained from Novus Biologicals Europe and R&D Systems Bio-techne, respectively. Other chemicals were purchased from Sigma or Calbiochem (Merck, UK).
Gryshchenko O., Gerasimenko J.V., Petersen O.H, & Gerasimenko O.V. (2020). Calcium Signaling in Pancreatic Immune Cells In situ. Function, 2(1), zqaa026.
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3

Pretreatment and LPS Stimulation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were pretreated for 1 h with drug treatment. ITI-214 was synthesized at Intra-Cellular Therapies, Inc. (Li et al., 2016 (link)). Other drugs were purchased from Sigma-Aldrich (ADP, IBMX, rolipram) or Tocris (AR-C 66096, and AR-C 69931). Where indicated, following pretreatment, LPS (from E. coli 0111:B4, Millipore-Sigma) was added to the media at 50 ng/mL for 4 h. At 5 h, the cells or media were harvested and processed as indicated below.
O'Brien J.J., O'Callaghan J.P., Miller D.B., Chalgeri S., Wennogle L.P., Davis R.E., Snyder G.L, & Hendrick J.P. (2019). Inhibition of calcium-calmodulin-dependent phosphodiesterase (PDE1) suppresses inflammatory responses. Molecular and cellular neurosciences, 102, 103449.
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