Blood samples were collected every three days (d1, 4, 7, 10, 13, 16, 19, 22, 25, and 28) at 1400 h from jugular venepuncture using non-heparinized vacutainers (20 mL; Becton–Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton–Dickinson, Franklin Lakes, NJ, USA)21 (link). Serum samples were obtained from blood after centrifugation at 2700× g at 4 °C for 15 min. Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at − 80 °C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PIII, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PIIIs, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
7600 chemistry autoanalyzer
Manufactured by Hitachi
Sourced in Germany, Japan
The HITACHI 7600 chemistry autoanalyzer is a laboratory instrument designed for automated analysis of chemical samples. It performs colorimetric and ion-selective measurements to determine the concentration of various analytes in a sample. The core function of the HITACHI 7600 is to provide efficient and reliable analysis of clinical chemistry parameters.
Automatically generated - may contain errors
Lab products found in correlation
1.5 ml tube, by Eppendorf (2 mentions)
Dri chem 7000i biochemistry analyzer, by Fujifilm (2 mentions)
Free fatty acids kits, by Roche (2 mentions)
Bovine elisa test kit, by Life Diagnostics (2 mentions)
Fuji dri chem slide glu p 3, by Fujifilm (1 mentions)
Spectramax 190, by Molecular Devices (1 mentions)
Architect c8000 system, by Abbott (1 mentions)
Cias latex crp h, by Kanto Chemical (1 mentions)
4 protocols using 7600 chemistry autoanalyzer
1
Serum Biomarker Analysis in Blood Samples
2
Urinary Biomarkers in Porcine Surgery
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A urine sample was obtained by a veterinarian (he is not an author) who was unaware of the surgical results, using a nelaton catheter at 6, 24, and 72 hours, postoperatively. Obtained urine samples were encoded and stored in a -80°C deep freezer until ELISA analysis. All ELISA analyses were performed by a researcher (she is not an author) who was unaware of details of the experiment. NGAL was measured using a pig NGAL ELISA kit (Bioporto, Hellerup, Denmark). Microalbumin was measured using a pig microalbumin ELISA kit (KAMIYA, Seattle, WA). KIM-1 was measured using a porcine KIM-1 ELISA kit (MyBioSource Inc., San Diego, CA). All ELISA analyses were measured with a SpectraMax 190 (Molecular Devices, Sunnyvale, CA). Because hydration status can affect the concentration of these urinary markers, urinary creatinine was used as a normalization variable. Urine creatinine was measured by a HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan).
3
Comprehensive Metabolic Profiling Protocol
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Laboratory tests were done at the central laboratory of the Kailuan General Hospital. Blood samples were collected from cubital veins and transfused into vacuum tubes containing EDTA after 8-h fasting, all biochemical variables were measured using Hitachi 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). The tests included: fasting plasma glucose (FBG), and triglyceride, total cholesterol (TC), HDL cholesterol (HDL-C); LDL cholesterol (LDL-C); uric acid; creatinine; high-sensitivity C reactive protein (hsCRP). FBG was measured by the hexokinase method (BioSino Bio-Technology & Science, Beijing, China). Cholesterol concentrations were directly measured by the Vertical Auto Profile (VAP; Atherotech, Birmingham, Alabama, USA), an inverted rate zonal, single-vertical spin, density-gradient ultracentrifugation technique that separates lipoprotein subfractions and then measures the cholesterol content, including LDL-C and HDL-C [18] . Triglyceride was directly measured using the ARCHITECT C-8000 system (Abbott, Abbott Park, Illinois, USA). Uric acid was measured by uric acid plus method (Biology Institute of China North, Beijing, China) and (hsCRP) was determined using a commercial, high-sensitivity particle-enhanced immunonephelometry assay (Cias Latex CRP-H; Kanto Chemical Co, Chuo-ku, Japan). Creatinine clearance (CCr) (ml/min) was calculated based on cockcroft-gault equation.
4
Comprehensive Blood Sampling and Analysis
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Blood samples were collected every three days (d1, 4, 7, 10, 13, 16, 19, 22, 25, and 28) at 1400 h from jugular venepuncture using non-heparinized vacutainers (20 mL; Becton-Dickinson, Belliver Industrial Estate, PL6 7BP, Plymouth, UK) and ethylenediaminetetraacetic acid-treated vacutainers (4 mL; Becton-Dickinson, Franklin Lakes, NJ, USA) 20 . Serum samples were obtained from blood after centrifugation at 2,700 × g at 4°C for 15 minutes.
Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
Serum was transferred to a 1.5 mL tube (Eppendorf AG, Hamburg, Germany) and kept at -80°C until further analysis. Serum was analyzed for glucose (Fuji Dri-Chem Slide Glu-PⅢ, Fuji Film Corp., Tokyo, Japan) and blood urea nitrogen (BUN, Fuji Dri-Chem Slide BUN-PⅢ, Fuji Film Corp., Tokyo, Japan) using DRI CHEM 7000i biochemistry analyzer (Fuji Film, Tokyo, Japan). Non-esterified fatty acids (NEFA) was analyzed using Roche Free Fatty Acids kits (Roche, Mannheim, Germany) with HITACHI 7600 chemistry autoanalyzer (Hitachi, Tokyo, Japan). Blood cortisol was determined using a commercial Bovine ELISA test kit (Life Diagnostics, Inc, West Chester, PA, USA). Plasma was deproteinized with 10% sulfosalicylic acid for analysis of AA profiles using the AA analyzer (Sykam S433, Sykam GmbH, Germany).
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