Dibasic sodium phosphate (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). From Alfa Aesar (Karlsruhe, Germany), 3(trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) was obtained. Deuterium oxide (D2O) was obtained from Cambridge Isotopes laboratories (Tewksbury, MA, USA). Deionized water was purified using the in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). All chemicals were used with no further purification. The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na2HPO4, 0.4 g of TSP, and 0.2 g NaN3 in 400 mL of D2O and adjusted to pH 7.4 with 1M NaOH and HCl. Upon addition of D2O to a final volume of 500 mL, the pH was re-adjusted to pH 7.4 with 1M NaOH and HCl.
Milli q advantage water purification system
Manufactured by Merck Group
Sourced in Germany
The Milli-Q Advantage Water Purification System is a laboratory equipment product that provides high-quality purified water. It utilizes a multi-stage filtration process to remove impurities, producing water that meets the specifications required for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Deuterium oxide d2o, by Cambridge Isotopes (4 mentions)
Sodium azide nan3, by Avantor (4 mentions)
3 trimethylsilyl propionic acid 2 2 3 3 d4 sodium salt tsp, by Thermo Fisher Scientific (4 mentions)
Hydrochloric acid (hcl), by Avantor (3 mentions)
Sodium phosphate, by Avantor (3 mentions)
Methanol, by Avantor (1 mentions)
4 protocols using milli q advantage water purification system
1
Preparation of Phosphate NMR Buffer
2
NMR Spectroscopy Sample Preparation
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Methanol, sodium phosphate, dibasic (Na2HPO4), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3-(Trimethylsilyl)propionic-2,2,3,3-d4 acid sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). Deionized water was purified using an in-house Milli-Q® Advantage water purification system from Millipore (Schwalbach, Germany).
3
Preparation of Phosphate NMR Buffer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium phosphate, dibasic (Na2HPO4), sodium hydroxide (NaOH), hydrochloric acid (HCl, 32% m/v), and sodium azide (NaN3) were obtained from VWR International (Darmstadt, Germany). 3(Trimethylsilyl) propionic acid-2,2,3,3-d4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D2O) was obtained from Cambridge Isotopes Laboratories (Tewksbury, MA, USA). Deionized water was purified using an in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). All chemicals were used without further purification. The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na2HPO4, 0.4 g of TSP, and 0.2 g NaN3, in 400 mL of D2O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D2O to a final volume of 500 mL the pH was re-adjusted to pH 7.4 with 1 M NaOH and HCl.
4
Preparation of NMR Phosphate Buffer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Sodium phosphate, dibasic (Na 2 HPO 4 ), sodium hydroxide, hydrochloric acid (32% m/v), and sodium azide (NaN 3 ) were obtained from VWR International (Darmstadt, Germany). 3(trimethylsilyl) propionic acid-2,2,3,3-d 4 sodium salt (TSP) was obtained from Alfa Aesar (Karlsruhe, Germany). Deuterium oxide (D 2 O) was obtained from Cambridge Isotopes laboratories (Tewksbury, MA). Deionized water was purified using in-house Milli-Q Advantage Water Purification System from Millipore (Schwalbach, Germany). The phosphate NMR buffer solution was prepared by dissolving 5.56 g of anhydrous Na 2 HPO4, 0.4 g of TSP, and 0.2 g NaN 3 , in 400 mL of D 2 O and adjusted to pH 7.4 with 1 M NaOH and HCl. Upon addition of D 2 O to a final volume of 500 mL, the pH was readjusted to pH 7.4 with 1 M NaOH and HCl.
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