We performed cell culture of cell lines in standard conditions in a humidified atmosphere at 37°C under 5% CO2. DND41 (#ACC-525; RRID:CVCL_2022), JURKAT (#ACC-282; RRID:CVCL_0065), HPB-ALL (#ACC-483; RRID:CVCL_1820) cells were obtained from Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ). We purchased HEK293T cells from American Type Culture Collection (ATCC #CRL-3216; RRID:CVCL_0063). No cell authentication was performed. T-ALL cells were cultured in HyClone RPMI 1640 Media (SH3002701, Fisher Scientific) supplemented with 10% FBS (900–108, Gemini Bio-Products) and 1% penicillin-streptomycin (45000–652, VWR).
Hyclone rpmi 1640 media
Manufactured by Thermo Fisher Scientific
HyClone RPMI 1640 Media is a commonly used cell culture medium. It is formulated to support the growth and maintenance of a variety of cell types. The media provides the necessary nutrients and components for cell proliferation and survival in an in vitro setting.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t cell, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Gemini Bio (1 mentions)
Jurkat, by Leibniz Institute DSMZ (1 mentions)
Penicillin streptomycin, by Avantor (1 mentions)
Calu 6, by American Type Culture Collection (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
Sk mes 1, by Corning (1 mentions)
Sk mes 1, by American Type Culture Collection (1 mentions)
Gibco keratinocyte sfm, by Thermo Fisher Scientific (1 mentions)
2 protocols using hyclone rpmi 1640 media
1
Cell Culture of T-Cell Leukemia Lines
2
Cell Culture of NSCLC Lines
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Non-small cell lung cancer cell lines (A549, Calu6, H358, H460 and SKMES-1) and non-tumorigenic bronchial epithelial cell line (BEAS-2B) were acquired from American Type Culture Collection (ATCC). The human bronchial epithelial cell line (HBEC) was a gift from John Minna’s lab. A549, Calu6, H358, and H460 were maintained in HyClone™ RPMI 1640 Media (Fisher Scientific, cat no. 16777-145), and SKMES-1 in Corning™ Eagle's Minimum Essential Medium (MEM) (cat no. MT10009CV). HBEC cells were maintained in Gibco™ Keratinocyte-SFM (Thermofisher Scientific, cat no. 17005042). NSCLC cell lines were supplemented with 10% Fetal Bovine Serum. FBS used for EV isolation (EV-depleted FBS) was prepared in 1:1 dilution with the respective media, centrifuged at 110,000xg for 18 h, and supernatant was processed through a Nalgene Thermo Rapid Flow 0.2 µm filter units (Fisher Scientific, cat.no 09-741-02). All cell lines were supplemented with 1% Cytiva HyClone™ Penicillin Streptomycin (Fisher Scientific, cat no. SV300-10) and maintained at 37 °C in 5% CO2 humidified incubator.
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