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2 protocols using methyl cinnamate

1

Quantification of Carbonyl Compounds and Amino Acids

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D-Glucose, D-fructose, D-maltose, D-lactose, L-lysine, L-serine, L-threonine, L-cysteine, L-norvaline, glyoxal, methylglyoxal, diacetyl, quinoxaline, 2-methylquinoxaline, 2,3-dimethylquinoxaline, o-phenylenediamine dihydrochloride, 1-methylpyrazole, C8-C20 alkane standard, methyl cinnamate, and sodium chloride were purchased from Sigma-Aldrich (St. Louis, MO). Water and ethyl acetate were obtained from J.T. Baker (Philipsburg, NJ, USA). All solutions were stored at 4 °C until analysis. Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS, 50 μm film thickness) solid phase microextraction (SPME) fiber, polypropylene hold caps, and polytetrafluoroethylene (PTFE)/silicone septa were purchased from Supelco Inc. (Belfonte, PA, USA).
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2

Enzymatic Synthesis of Methyl Cinnamate

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Immobilized lipase Novozym® 435 (10000 PLU g−1; propyl laurate units)—from Candida antarctica B (EC 3.1.1.3), supported on a macroporous acrylic resin—was purchased from NovoNor disk Bioindustrials Inc. (Copenhagen, Denmark). Methyl cinnamate was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Further, 1-octanol was purchased from Showa Chemical Industry Co., Ltd. (Tokyo, Japan). Molecular sieve 4 Å was obtained from Davison Chemical (Baltimore, MD, USA). All chemicals used were of analytical reagent grade.
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