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Islet cell tumor nbp2 77922

Manufactured by Novus Biologicals
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The Islet Cell Tumor NBP2–77922 is a laboratory product used for research purposes. It is a reagent intended for use in various experimental techniques, such as immunohistochemistry, immunocytochemistry, and related assays. The product is derived from a specific type of tumor cell line.

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Lab products found in correlation

Ab134152, by Abcam (2 mentions) Lsm 8800 confocal microscope, by Zeiss (2 mentions) Af594, by Thermo Fisher Scientific (2 mentions) Alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Umb 1, by Abcam (1 mentions) γh2ax, by Merck Group (1 mentions)

2 protocols using islet cell tumor nbp2 77922

1

Immunofluorescence Staining of Tumor Samples

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Immunofluorescence (IF) staining was performed on commercially available pancreas tissue slides (Islet Cell Tumor NBP2–77922, Novus Biologicals), as a positive control for SSTR2, and on formalin-fixed, paraffin-embedded (FFPE) mouse tumor samples. Slides were incubated with the primary antibody for SSTR2 (UMB1; ab134152; 1:25; Abcam) and a secondary antibody, Alexa Fluor 594 (A11037;1:500, Invitrogen); For Pan-acetylated H3 primary antibody (06–599; 1:500; EMD Millipore) and secondary antibody (AF594; 1:100, Thermo Fisher Scientific); For γH2AX primary antibody (05–636–1; 1:800; EMD Millipore) and secondary antibody (AF488; 1:100, Thermo Fisher Scientific; Supplementary Table S1). Zeiss LSM - 8800 Confocal microscope was used for images.
Sharma R., Earla B., Baidoo K.E., Zeiger M.A., Madigan J.P., Escorcia F.E, & Sadowski S.M. (2023). Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model. Molecular Cancer Therapeutics, 22(9), 1052-1062.
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2

Immunofluorescence Staining of Pancreas and Mouse Tumors

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Immunofluorescence (IF) staining was performed on commercially available pancreas tissue slides (Islet Cell Tumor NBP2-77922, Novus Biologicals), as a positive control for SSTR2, and on formalin-fixed, paraffin-embedded mouse tumor samples. Slides were incubated with the primary antibody for SSTR2 (UMB1) (ab134152; 1:25; Abcam) and a secondary antibody, Alexa Fluor 594 (A11037;1:500, Invitrogen); For Pan-acetylated H3 primary antibody (06-599; 1:500; EMD Millipore) and secondary antibody (AF594; 1:100, Thermo Fisher Scientific); For γH2AX primary antibody (05-636-1; 1:800; EMD Millipore) and secondary antibody (AF488; 1:100, Thermo Fisher Scientific) (Supplementary Table 1). Zeiss LSM - 8800 Confocal microscope was used for images.
Sharma R., Earla B., Baidoo K.E., Zeiger M.A., Madigan J.P., Escorcia F.E, & Sadowski S.M. (2023). Upregulation of Somatostatin Receptor Type 2 Improves 177Lu-DOTATATE Therapy in Receptor-Deficient Pancreatic Neuroendocrine Tumor Model. Molecular Cancer Therapeutics, 22(9), 1052-1062.
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