Pacgfp1 c1

Manufactured by Addgene

Sourced in United States

PAcGFP1-C1 is a plasmid vector that contains the gene encoding the Aequorea coerulescens green fluorescent protein (AcGFP1). It is designed for expression and detection of proteins fused to the C-terminus of AcGFP1 in mammalian cells.

Automatically generated - may contain errors

Lab products found in correlation

Pgbw m4046887, by Addgene (1 mentions) Nebuilder hifi dna assembly cloning kit, by New England Biolabs (1 mentions) Plvx tre3g, by Takara Bio (1 mentions)

2 protocols using pacgfp1 c1

Cloning SARS-CoV-2 Nsp12 and S Protein

Check if the same lab product or an alternative is used in the 5 most similar protocols

Nsp12 and S protein genes of SARS2 virus were amplified from plasmids pGBW-m4046955 (no. 145616) and pGBW-m4046887 (no. 145730) (Addgene), respectively, and cloned into plasmid pAcGFP1-C1 (no. 121046) (Addgene) by using the NEBuilder HiFi DNA assembly cloning kit (NEB, catalog no. E5520S) following the manufacturer’s instructions. Plasmid pAcGFP1-C1 was linearized by double digestion of KpnI and BamHI. The primers used to generate construction are listed in Table S3. Positive colonies were confirmed by PCR and Sanger sequencing.

Teng Y., Xu F., Zhang X., Mu J., Sayed M., Hu X., Lei C., Sriwastva M., Kumar A., Sundaram K., Zhang L., Park J.W., Chen S.Y., Zhang S., Yan J., Merchant M.L., Zhang X., McClain C.J., Wolfe J.K., Adcock R.S., Chung D., Palmer K.E, & Zhang H.G. (2021). Plant-derived exosomal microRNAs inhibit lung inflammation induced by exosomes SARS-CoV-2 Nsp12. Molecular Therapy, 29(8), 2424-2440.

+ Open protocol

+ Expand

Generating GFP-SEC24D Fusion Construct

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human SEC24D was cloned from pJK15 plasmid into pAcGFP1-C1 (Addgene, Watertown, MA, USA) to generate a GFP-SEC24D fusion gene. GFP-SEC24D sequence was amplified with PCR using primers described in Table S1. These PCR products were used to insert GFP-SEC24D into a lentivirus vector (pLVX-TRE3G) through In-Fusion Cloning, following manufacturer’s protocol (Takara Bio, Mountain View, CA, USA). Lentivirus vectors pLVX-Tet3G and pLVX-TRE3G (Cat. 631358 and 631193) were purchased from Takara Bio.

Lu C.L., Ortmeier S., Brudvig J., Moretti T., Cain J., Boyadjiev S.A., Weimer J.M, & Kim J. (2021). Collagen has a unique SEC24 preference for efficient export from the endoplasmic reticulum. Traffic (Copenhagen, Denmark), 23(1), 81-93.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!