ARA, EPA, and DHA were purchased from Nacalai Tesque (Kyoto, Japan). (±)8-HEPE (comprising equal amounts of 8S-HEPE and 8R-HEPE), 8S-HEPE, (±)8-HETE, 8S-HETE, 8R-HETE, rac-10-HDHA, PD1, gamma-linoleic acid (GLA), stearidonic acid (SDA) and docosapentaenoic acid (DPA) were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). The Spodoptera frugiperda (Sf9) insect cells, and pFastBac vector were purchased from Thermo Fisher Scientific (Franklin, MA, USA). The anti-6xHis antibody [HIS.H8] was purchased from Abcam Japan (Tokyo, Japan).
Yuki S., Uemura A., Hakozaki M., Yano A., Abe M., Misawa Y., Baba N, & Yamada H. (2020). Identification of a novel enzyme from E. pacifica that acts as an eicosapentaenoic 8R-LOX and docosahexaenoic 10R-LOX. Scientific Reports, 10, 20592.
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