After treating neutrophils with PBS (vehicle control) or recombinant a2NTD or TCM for indicated time points incubation at 37 °C, 5% CO2 incubator, total RNA was isolated using RNeasy micro kit (Qiagen) and single-stranded cDNA was synthesized using QuantiTect reverse transcription kit (Qiagen). Qantitative real-time PCR (Q RT-PCR) was performed, using cDNA specific FAM-MGB–labeled Taqman primer sets (Applied Biosystems) for IL-8 gene and VIC-MGB labeled 18s rRNA was used as endogenous control.
Fam mgb labeled taqman primer sets
Manufactured by Thermo Fisher Scientific
FAM-MGB–labeled Taqman primer sets are a type of real-time PCR assay designed for gene expression analysis. These primer sets utilize a fluorescent reporter dye (FAM) and a minor groove binder (MGB) to enable sensitive and specific detection of target sequences.
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Lab products found in correlation
2 protocols using fam mgb labeled taqman primer sets
1
Analyzing IL-8 Gene Expression in Neutrophils
2
Quantification of Gene Expression
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After treating neutrophils with PBS (control) or recombinant a2NTD for 4 hr incubation, total RNA was isolated using RNeasy micro kit (Qiagen, Germantown, MD) and single-stranded cDNA was synthesized using QuantiTect reverse transcription kit (Qiagen). Qantitative real-time PCR (Q RT-PCR) was performed, using cDNA specific FAM-MGB–labeled Taqman primer sets (Applied Biosystems) for various genes and VIC-MGB labeled 18s rRNA was used as endogenous control.
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