Total RNA was isolated from tissues or cells using TRNzol (TianGen, Beijing, People’s Republic of China). For mRNAs and lincRNA quantification, the reverse transcription was performed using HiScript® II Reverse Transcriptase (Vazyme, Nanjing, People’s Republic of China) in accordance with manufacturer’s instructions. Quantitative real-time PCR was performed using specific primers with GAPDH as an internal control. For miRNA quantification, reverse transcription was performed using miRNA First-Stand cDNA Synthesis Kit (GeneCopoeia, Guangzhou, People’s Republic of China) with specific primers for miRNA-143-3p and U6. Primer sequences were provided in Supplementary Table S1 Supplementary Table S1
Mirna first stand cdna synthesis kit
Manufactured by GeneCopoeia
Sourced in China
The MiRNA First-Strand cDNA Synthesis Kit is a lab equipment product designed for the reverse transcription of microRNA (miRNA) into complementary DNA (cDNA). It provides the necessary reagents and protocols for the efficient conversion of miRNA to cDNA, which is a crucial step in various miRNA analysis and profiling applications.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (3 mentions)
Applied biosystems 7500 real time pcr system, by Thermo Fisher Scientific (3 mentions)
Cdna synthesis supermix kit, by Transgene (2 mentions)
Trnzol, by Tiangen Biotech (1 mentions)
Hiscript 2 reverse transcriptase, by Vazyme (1 mentions)
Primescript rt master mix kit, by Takara Bio (1 mentions)
4 protocols using mirna first stand cdna synthesis kit
1
Quantification of RNA Species
2
Quantification of MIR100HG and miR-5590-3p
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNAs were extracted from tissues and cells using Trizol reagent (Invitrogen). For the quantification of MIR100HG with DCBLD2, reverse‐transcription was performed using PrimeScript RT Master Mix kit (TaKaRa) as per the manufacturer's instructions, with GAPDH as the internal reference gene. For miR‐5590‐3p, miRNA First‐Stand cDNA Synthesis Kit (GeneCopoeia) was used with U6 as the internal reference gene. PCR primers were designed and synthesized by Sangon (The primer sequences were listed in Table 2 ). Real‐time PCR reactions were performed on Applied Biosystems 7500 Real‐time PCR Systems (Thermo Fisher Scientific). The 2−ΔΔCt method was used to normalize the data, and the experiment was repeated three times.
3
Quantitative Expression Analysis of miRNAs and lncRNAs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
First, TRIzol reagent (Invitrogen) was used to isolate total RNAs from OC cell lines. Next, the reverse transcription of miRNA or lncRNA/mRNA was performed by the miRNA First-Stand cDNA Synthesis Kit (GeneCopoeia) or the cDNA Synthesis SuperMix Kit (TransGen, Beijing, China). Afterwards, RT-qPCR was performed on Applied Biosystems 7500 Real-time PCR Systems (Thermo Fisher Scientific). Gene expression was quantified by 2−ΔΔCt method and normalized to U6 or GAPDH.
4
miRNA and lncRNA/mRNA Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Firstly, Trizol reagent (Invitrogen) was used to isolate total RNAs from OC cell lines. Then, the reverse transcription of miRNA or lncRNA/mRNA was performed by miRNA First-Stand cDNA Synthesis Kit (GeneCopoeia) or cDNA Synthesis SuperMix Kit (TransGen, Beijing, China). Afterwards, RT-qPCR was performed on Applied Biosystems 7500 Real-time PCR Systems (Thermo Fisher Scienti c). Gene expression was quanti ed by 2 -ΔΔCt method normalized to U6 or GAPDH.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!