Dual luciferase reporter system detection kit

Manufactured by Promega

The Dual-Luciferase Reporter System Detection kit is a laboratory tool used to measure the activities of two different luciferase reporter enzymes simultaneously in a single sample. The kit includes reagents necessary for the quantitative analysis of firefly and Renilla luciferase activities.

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2 protocols using dual luciferase reporter system detection kit

Dual-Luciferase Reporter Assay Protocol

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Luciferase assays were performed using the Dual-Luciferase Reporter System Detection kit (Promega). 48 hours post transfection, cells were washed with PBS and lysed. The cell lysate was centrifuged to remove cellular debris. 10 μl of the cleared lysate was used for the simultaneous detection of firefly (Fluc) and renilla (Rluc) luciferases with a Luminometer (Promega). Reporter values were normalized and presented as the ratio of Fluc/Rluc.

Lin J., Gu C., Shen Z., Liu Y., Wang W., Tao S., Cui X., Liu J, & Xie Y. (2017). Hepatocyte nuclear factor 1α downregulates HBV gene expression and replication by activating the NF-κB signaling pathway. PLoS ONE, 12(3), e0174017.

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Promoter Reporter Assay with Dual-Luciferase

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The Dual-Luciferase Reporter System Detection kit (Promega) was used for the promoter reporter assays. Cells were cultured in 24-well plates and transfected with 0.4 μg of one of the promoter reporter plasmids (Cp, Sp1, Sp2 and Xp) and 0.1 μg pRL-TK per well. At 48 h post-transfection, cells were washed with PBS and lysed in 100 μl lysis buffer. Cell lysates were centrifuged to remove cellular debris. Firefly and Renilla luciferase activities in cell lysate were detected with a Luminometer (Promega), according to the manufacturer's instructions.

Song Y., Shou S., Guo H., Gao Z., Liu N., Yang Y., Wang F., Deng Q., Liu J, & Xie Y. (2022). Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. Virologica Sinica, 37(4), 558-568.

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