N 2 6 dimethylphenylcarbamoylmethyl triethylammonium bromide qx 314

All chemical compounds used in extracellular and intracellular solutions as well as 4-aminopyridine (4-AP), ouabain octahydrate (ouabain), and nifedipine were obtained from Sigma-Aldrich Sweden AB (Stockholm, Sweden). Kainic acid (KA), (E)-capsaicin (Cp), capsazepine (Cz), d-(-)-2-amino-5-phosphonopentanoic acid (AP5), 6,7-dinitroquinoxaline-2,3-dione disodium salt (DNQX), picrotoxin, N-(2,6-Dimethylphenylcarbamoylmethyl)-triethylammonium bromide (QX-314), and tetrodotoxin citrate (TTX) were obtained from Tocris Bioscience (Bristol, UK). Ouabain was dissolved in standard ACSF (composition see below) and incubated 1 h at 37 °C before use. 4-AP, nifedipine, Cp, Cz, and picrotoxin were dissolved in DMSO 100%. AP5, DNQX, TTX, and KA were dissolved in milliQ water.

Picrotoxin, 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxa-line (NBQX), 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and N-(2,6-dimethylphenylcarbamoylmethyl)triethylammonium bromide (QX314) were purchased from Tocris (R&D System Inc., Minneapolis, MN, USA). D-2-amino-5-phosphonopentanoic acid (D-AP5) and tetrodotoxin (TTX) were obtained from Abcam, Inc.