Each sequence‐unique strain (N = 39) was cultured on lysogeny broth agar at 30°C for 48–72 h and inoculated into Biolog IF‐A Inoculating Fluid to a 90%–98% transmittance measured by a Biolog turbidimeter (Biolog, Inc. Hayward, CA). Aliquots (100 µl) of IF‐A suspensions were incubated for 24 h on GEN III MicroPlates and optical density at 550 nm (OD550) of each well was measured on a BioTek Gen5 plate reader (BioTek Instruments, Inc., Winooski, VT). Two of the 39 strains were unable to grow in IF‐A and thus were not scored. Biolog plates come with a negative control well with no carbon sources and a positive control well that reacts with the tetrazolium dye contained in the wells to provide a baseline for comparison. Stain‐specific OD550 was standardized by subtracting the negative control and dividing by the positive control.
If a inoculating fluid
Manufactured by Biolog
IF-A Inoculating Fluid is a sterile, buffered aqueous solution designed for the standardization of bacterial suspensions for various microbiological applications. The product provides a consistent medium for the preparation of inocula, ensuring reliable and reproducible results.
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Lab products found in correlation
2 protocols using if a inoculating fluid
1
Biolog GEN III Microbial Phenotyping
2
Physiological Profiling of Bacterial Strains
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Biolog GEN III plates (Cat. No. 1030) were used to ascertain the physiological and biochemical characteristics of strain SyEd1 T in vitro, and these were conducted alongside Pectobacterium carotovorum subsp. carotovorum LMG 02404 T for comparison. Within this, we also performed the assay in the presence/absence of 0.4 % polygalacturonic acid (PGA; Sigma, P3850), which is commonly used to induce the expression of plant cell wall-degrading enzymes (for preparation of PGA, see [17] . For the Biolog GEN III assays, we used IF-A inoculating fluid (Biolog, Cat. No. 72401) with or without PGA supplementation to a final concentration of 0.4 % PGA. Both bacterial species were grown overnight, diluted to an OD 600 =0.4 and 50 µl of this aliquot were added to a tube containing IF-A fluid. The aliquot in the IF-A tube was homogeneously mixed using a vortex and 100 µl of this suspension was added to each of the 96 wells of the Biolog GEN III plate. The plate was subsequently incubated at 30 °C without shaking.
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