Rrx 001

Human small cell lung cancer cells (H82, SHP77, H526, H69, and DMS273) were kindly provided by Dr. Matthew Meyerson’s Laboratory at Dana-Farber Cancer Institute, USA. H82, SHP77, H526, H69 are originally purchased from the American Type Culture Collection (ATCC) and DMS273 was originally obtained from the European Collection of Cell Cultures (ECACC).
All human small cell lung cancer cells (H82, SHP77, H526, H69, and DMS273) were cultured at 37 °C in a humid atmosphere containing 5% carbon dioxide and in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS). JQ1 was purchased from Selleck Chemical (Shanghai, China), and acetylcysteine (NAC), ATRA, and RRx-001 were obtained from MedChemExpress (Shanghai, China). JQ1, ATRA, and RRx-001 were dissolved and aliquoted in DMSO (Sigma-Aldrich, Shanghai, China) and NAC was diluted in nuclease-free water.

Chitosan, Pluronic F127,1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), N-hydroxysuccinimide (NHS), succinic anhydride, 4-morpholineethanesulfonic acid (MES), 4-dimethylaminopyridine (DMAP), indocyanine green (ICG) and RRx-001 were purchased from Sigma–Aldrich and MCE (United States). Primary antibodies, including anti-G6PD, anti-P21, anti-P53, anti-Bcl-2, anti-MMP2, anti-ZEB1 and anti-HIF-1α, were purchased from Abcam (Cambridge, United Kingdom). The 4′,6-diamidino-2-phenylindole (DAPI), Calcein AM, propidium iodide (PI), 2,7-dichlorodihydrofluorescein diacetate (DCF), TUNEL assay kit and BCA kit were purchased from Thermo Fisher Scientific (United States).