2 3 dimethoxy 5 methyl p benzoquinone q0

Manufactured by Merck Group

Sourced in Germany

2,3-dimethoxy-5-methyl-p-benzoquinone (Q0) is a chemical compound used in various laboratory applications. It is a benzoquinone derivative with specific structural and functional properties. The core function of this compound is to serve as a research tool in scientific investigations, without extrapolation on its intended use.

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Benzoquinone (42 citations) P-benzoquinone (41 citations)

3 protocols using 2 3 dimethoxy 5 methyl p benzoquinone q0

Transient Absorption Spectroscopy of Photosynthetic Complexes

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Samples for TA measurements were
prepared in a quartz cuvette with a 2 mm optical path length (Gallab)
from stock solutions diluted in 20 mM Tris (pH 8.0)/0.1% LDAO directly
prior to the TA experiment. Stock solutions used were 10.0 ±
0.8 mg/mL polymer (either P-Os or PVI), 20.9 ± 1.4 mg/mL RCs,
15 mM 2,3-dimethoxy-5-methyl-p-benzoquinone (Q₀; Sigma-Aldrich), and 1 M sodium ascorbate (Sigma-Aldrich).
Five experimental samples (named I–V) were prepared from stock
solutions of RCs, P-Os, and Q₀, along with several control
samples, as detailed in Table 1. Where present, the concentration of Q₀ was kept
constant at around 1.5 mM to enable efficient electron acceptance
from RCs.^{28 (link)} More detailed information on
the sample composition and preparation is given in Section S2 of the SI.
Solutions for steady-state
experiments with centrifugation were
prepared as for samples I–V for TA measurements but without Q₀ (the same amount of
pure H₂O was used instead).

Białek R., Thakur K., Ruff A., Jones M.R., Schuhmann W., Ramanan C, & Gibasiewicz K. (2020). Insight into Electron Transfer from a Redox Polymer to a Photoactive Protein. The Journal of Physical Chemistry. B, 124(49), 11123-11132.

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Detailed Reagent and Buffer Protocol

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N-tris-(hydroxymethyl)-methyl-3-aminopropanesulfonic acid (TAPS buffer), 3-(N-morpholino)propanesulfonic acid (MOPS buffer), 2-(N-Morpholino)ethanesulfonic acid 4-Morpholineethanesulfonic acid (MES buffer), 3,3’,5,5’-tetramethylbenzidine (TMB), guaiacol, 2,3-Dimethoxy-5-methyl-p-benzoquinone (Q0), sodium fluoride, sodium acetate, ammonium persulfate, sodium dithionite, and L-tyrosine, were obtained from Sigma-Aldrich (Steinheim, Germany). 2-Amino-2-hydroxymethyl-propane-1,3-diol (TRIS buffer) and potassium ferricyanide were purchased from Merck (Darmsstadt, Germany). Guanidine hydrochloride was purchased from Alfa-Aesar (Karlsruhe, Germany). Water-free acetic acid and ethanol were purchased from Nordic Invest (Cluj-Napoca, Romania) and sodium chloride from Poch (Gliwice, Poland), while ammonium sulfate was purchased from Lach-ner (Brno, Czech Republic). The compounds and solvents were of HPLC or analytical degree purity. For buffers and washing, ultra-pure water (0.055 μS/cm) obtained via an SG ultra-pure water system (Barsbüttel, Germany) was used. 2,2-Azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) was purchased from TCI-SU (Tokyo, Japan).

Mot A.C., Coman C., Hadade N., Damian G., Silaghi-Dumitrescu R, & Heering H. (2020). “Yellow” laccase from Sclerotinia sclerotiorum is a blue laccase that enhances its substrate affinity by forming a reversible tyrosyl-product adduct. PLoS ONE, 15(1), e0225530.

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Electrochemical Analysis of Horse Heart Cytochrome c

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Horse heart cyt c, 2,3-dimethoxy-5-methyl-p-benzoquinone (Q₀), mercaptoundecanoic acid,
and mercaptoundecanol were purchased from Sigma-Aldrich. Milli-Q water
(Millipore, MA) was used in all preparations and procedures. Planar
disc 2 mm Ag electrodes were purchased from CH Instruments, Austin,
TX. Reference electrodes, counter electrodes, and potentiostats were
purchased from Metrohm Autolab BV, Utrecht, Netherlands. A high-power
multiarray LED (870-66-60) centered at 870 nm was purchased from Roither-Lasertechnik
GmbH, Wien, Austria.

van Moort M.R., Jones M.R., Frese R.N, & Friebe V.M. (2023). The Role of Electrostatic Binding Interfaces in the Performance of Bacterial Reaction Center Biophotoelectrodes. ACS Sustainable Chemistry & Engineering, 11(7), 3044-3051.

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