Df131

Plasma parameters were measured on two-times diluted samples (1:1 ratio of plasma to diluent) using DimensionXpand Plus (Siemens Healthcare Diagnostics). The biochemical tests were performed according to the manufacturer kit for each parameter: enzymatic creatinine (DF270B; Siemens Healthcare), glucose (DF40; Siemens Healthcare), high-density lipoprotein (DF48B; Siemens Healthcare), LDL (DF131; Siemens Healthcare), cholesterol (DF27; Siemens Healthcare), transaminase ASAT (DF41A; Siemens Healthcare), transaminase ALAT (DF143; Siemens Healthcare), urea nitrogen (DF21; Siemens Healthcare), and triglycerides (DF69A; Siemens Healthcare).
Plasma levels of TIMP-1, FGF-21, and GDF-15 were measured using the Mouse Premixed Multi-Analyte Kit (LXSAMSM; R&D Systems) in a Luminex 200 system following the manufacturer’s instructions.

Lipid profiles were measured by using Dimension^® Xpand^® Plus (Siemens Healthcare Diagnostics, Newark, DE, USA). The biochemical tests were performed according to the manufacturer instructions for each parameter; Cholesterol (Siemens Healthcare, DF27), TG (Siemens Healthcare, DF69A), LDL (Siemens Healthcare, DF131), and HDL (Siemens Healthcare, DF48B). AST and ALT were tested by using BioLis 24i Premium Chemistry Analyzer (BioRex Mannheim Malaysia Sdn. Bhd). The assay was performed according to the manufacturer instructions for each parameter; AST (aspartate aminotransferase EC.2.6.11 according to the International Federation of Clinical Chemistry and Laboratory Medicine [IFCC]) and ALT (alanine aminotransferase EC.2.6.11 according to IFCC). All procedures were conducted attentively and precisely according to the manufacturer’s instructions.

Blood was collected in a sterile tube. The blood was centrifuged at 3000 rpm at 4 °C for 10 min, and the serum was stored at −80 °C. Serum lipid profile was measured by using Dimension^® Xpand^® Plus (Siemens Healthcare Diagnostics, Newark, DE, USA). For each parameter, the evaluations were conducted based on manufacturer instructions; total cholesterol (Siemens Healthcare, DF27), triglyceride (Siemens Healthcare, DF69A), low-density lipoprotein (Siemens Healthcare, DF131), and high-density lipoprotein (Siemens Healthcare, DF48B). The HMG-CoA reductase was evaluated using a rat HMG-CoA ELISA kit (Wuhan Fine Biotech Co., Ltd., Wuhan, China). All procedures were conducted attentively and precisely according to the manufacturer’s instructions.