Cells were fixed with 4% paraformaldehyde (Sigma), followed by membrane permeabilization and blocking with 0.2% Triton X-100 (Sigma) and 2% donkey serum (Jackson ImmunoResearch Laboratories). All quantifications were performed blinded to genotype in ImageJ. Antibodies: Nestin (R&D MAB1259), TBR2 (Abcam ab23345), PAX6 (Covance PRB-278P), BRN2 (Abcam Ab137469), PKCλ (BD Transduction Laboratory 610207), Cleaved caspase 3 (R&D Systems AF835), TUNEL (Sigma 12156792910), Acetylated alpha-tubulin (Cell Signaling 5335S), P73 (Abcam Ab40658), and Reelin (Abcam AB5364).
Acetylated alpha tubulin
Manufactured by Cell Signaling Technology
Acetylated alpha-tubulin is a post-translationally modified form of the microtubule subunit alpha-tubulin. It is a component of the cytoskeleton and plays a role in cellular structure and function.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (2 mentions)
Reelin, by Abcam (1 mentions)
Nestin, by R&D Systems (1 mentions)
Donkey serum, by Jackson ImmunoResearch (1 mentions)
Cleaved caspase 3, by R&D Systems (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Alpha tubulin, by LI COR (1 mentions)
Phospho γh2a x, by Abcam (1 mentions)
Paraformaldehyde (pfa), by Santa Cruz Biotechnology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
2 protocols using acetylated alpha tubulin
1
Immunostaining for Neural Development
2
Multiparameter Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunofluorescence staining was performed according to standard protocols as described previously (Al-Khadairi et al., 2019) . Briefly, 80,000 cells were plated on Poly-Lysine coated glass coverslips (Corning), fixed with 4% paraformaldehyde (ChemCruz) and permeabilized with 0.1% Triton X-100 (Sigma). Primary antibodies against β-tubulin (Cell Signaling), phospho-γH2AX (Abcam), alpha-tubulin (Li-Cor), acetylated alpha-tubulin (Cell Signaling), MAP1B
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