Rabbit anti trpv1

Manufactured by Alomone

Rabbit anti-TRPV1 is a primary antibody that specifically recognizes the transient receptor potential vanilloid 1 (TRPV1) protein. TRPV1 is a non-selective cation channel that is activated by heat, protons, and certain vanilloid compounds. The rabbit anti-TRPV1 antibody can be used for the detection and analysis of TRPV1 expression in various experimental systems.

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Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Cresyl violet, by Merck Group (1 mentions) Mouse anti gfap, by Merck Group (1 mentions) Cy3 conjugated anti rabbit igg, by Merck Group (1 mentions) 3 3 diaminobenzidine (dab), by Merck Group (1 mentions) Bright field microscope, by Olympus (1 mentions) Vectashield containing dapi, by Vector Laboratories (1 mentions) Fluorescent microscope, by Olympus (1 mentions)

Spelling variants of this product

TRPV1 (11 citations) Anti-TRPV1 (6 citations) Rabbit anti-TRPV1 antibody (2 citations)

2 protocols using rabbit anti trpv1

Immunohistochemical Profiling of Brain Tissue

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Animals were transcardially perfused, fixed and brain tissues (40 µm thick) and processed for immunohistochemical staining. In brief, sections were rinsed in phosphate-buffered saline (PBS) then incubated with the following primary antibodies: rabbit anti-TRPV1 (1:1000, Alomone labs) and rabbit anti-CNTF (1:200, Santa-Cruz), mouse anti-GFAP (1:500, mouse, Sigma) for astrocytes and rabbit anti-TH (1:2000, rabbit, Pel-Freez) for dopamine neurons. 3,3'-diaminobenzidine (DAB; Sigma) was used to visualize TH⁺ cell and fiber. 1% Cresyl violet (Sigma) solution was used for Nissl staining. The next day, tissues were rinsed and incubated with FITC-conjugated-anti-mouse goat IgG (1:400, Millipore) and/or Cy3-conjugated-anti-rabbit IgG (1:400, Millipore) for 1 h. The stained tissues were viewed using a confocal microscopy (LSM 700, Carl Zeiss) or were analyzed under a bright-field microscope (Olympus).

Kim K.I., Baek J.Y., Jeong J.Y., Nam J.H., Park E.S., Bok E., Shin W.H., Chung Y.C, & Jin B.K. (2019). Delayed Treatment of Capsaicin Produces Partial Motor Recovery by Enhancing Dopamine Function in MPP+-lesioned Rats via Ciliary Neurotrophic Factor. Experimental Neurobiology, 28(2), 289-299.

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Immunohistochemical Analysis of Mouse Skin and DRG

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Dissected mouse skin or dorsal root ganglion (DRG) tissue were fixed in 4% paraformaldehyde at 4°C for 2 hours, cryoprotected in 25% sucrose in PBS for 24 hours and embedded in OCT at −80°C. DRG and skin tissue were cut at 12 uM and mounted onto Superfrost plus slides. Primary antibodies used included: goat anti-GFRα2 (R&D Systems, Minneapolis, MI), goat anti-GFRα3 (R&D Systems, Minneapolis, MI), rabbit anti-PGP9.5 (Ultraclone Ltd., Yarmouth, Isle of Wight), rabbit anti-TRPV1 (Alomone Labs, Jerusalem, Israel). Secondary antibodies used were: IB₄-Cy5 (Molecular Probes/Invitrogen Corporation), Cy2-conjugated donkey anti-goat (ImmunoResearch Laboratories, Inc., West Grove, PA), Alexa555-conjugated goat anti-rabbit (Invitrogen Corporation, Carlsbad, CA). Antibodies were diluted in antibody diluting buffer (1% BSA, 0.1% Tween20, 0.1% NaN3 in PBS). Tissues were mounted with Vectashield containing DAPI (Vector Laboratories, Burlingame, CA). Images were acquired using an Olympus fluorescent microscope (Olympus Corporation, Tokyo, Japan)

Cohen J.A., Edwards T.N., Liu A.W., Hirai T., Jones M.R., Wu J., Li Y., Zhang S., Ho J., Davis B.M., Albers K.M, & Kaplan D.H. (2019). Cutaneous TRPV1+ Neurons Trigger Protective Innate Type-17 Anticipatory Immunity. Cell, 178(4), 919-932.e14.

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