Aluminum chloride hydrate

DMEM, low-glucose (Dulbecco’s modified eagle’s medium), FBS (fetal bovine serum), penicillin-streptomycin (10×) solution, and PBS (phosphate-buffered saline) were purchased from Biological Industries, Haemek, Israel. Aluminum chloride hydrate, chromium (III) chloride hexahydrate, letrozole, exemestane, testosterone, DMSO (dimethyl sulfoxide), deionized water, and ethanol were obtained from Sigma Aldrich, Burlington, MA, USA. TrypLE^TM Express and GlutaMAX^TM were from Gibco, Waltham, MA, USA. Annexin V-FITC Apoptosis Kit and FxCycle^TM PI/RNase Staining Solution were purchased from Invitrogen, Waltham, MA, USA. Cell Proliferation Kit II (XTT) was from Roche Diagnostics, Mannheim, Germany. The Halt^TM Protease Inhibitor Coctail (100×) and Pierce^TM BCA Protein Assay Kit were obtained from Thermo Fisher Scientific, Waltham, MA, USA. The lysis buffer for ELISA, Nori Human Apoptosis Regulator BAX ELISA Kit, and Nori Human Bcl-2 ELISA Kit were purchased from Genorise Scientific, Glen Mills, PA, USA. Accutase^TM Cell Detachment was obtained from BD Biosciences, San Jose, CA, USA.