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Anti pka c alpha

Manufactured by Cell Signaling Technology

The Anti-PKA C-alpha is a laboratory reagent that targets the catalytic subunit alpha of Protein Kinase A (PKA). This antibody is designed to detect and monitor the expression of PKA C-alpha in various cellular and experimental settings.

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2 protocols using anti pka c alpha

1

Characterizing Kinase Signaling Pathways

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The following reagents and materials were used. Anti-p38 MAPK (ref. 9212), anti-phospho-p38 MAPK (T180/Y182) (ref. 9211), anti-Akt (ref. 4685), anti-phospho-Akt (S473) (ref. 4060), anti-PKA C-alpha (ref. 4782), anti-phospho-PKA C (T197) (ref. 5661), anti-SEK1 (ref. 9152), anti-phospho-SEK1 (S257/T261) (Ref. 9156), anti-MEK1/2 (ref. 9126), anti-phospho-MEK1/2 (S217/221) (ref. 9154), anti-phospho GSK3 α/β (S21) (ref. 9331), anti-GSK3 α/β (ref. 5676), anti-Phb1 (ref. 2426), and anti-Phb2 (ref. 14085) anti-PDK1 (ref. 3062), anti-phospho-PDK1 (S241) (ref. 3061), anti-phospho-PKC pan (T514) (ref. 9379), anti-MEK1/2 (ref. 9126), anti-phospho-MEK1/2 (S217/221) (ref. 9154), anti-phospho CAMKII (Y86) (ref.12716) and anti-CAMKII (ref.11945) were purchased from Cell Signaling Technology. Anti-PKC-pan was from Sigma Aldrich (ref. SAB4502356). Electrophoresis reagents were purchased from Bio-rad and trypsin from Promega.
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2

Comprehensive Signaling Pathway Analysis

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The following reagents and materials were used: anti-GAPDH (Calbiochem), anti-PKC-Pan, anti-pPKC-pan (T514), anti-pAkt (Ser473), anti-Akt, anti-cJUN, anti-phospho cJUN (S73), anti-NF-κB p65, anti-NF-κB phospho-p65 (S536), anti-IκB-alpha, anti-MEK, anti-pMEK (S217/221), anti-PKAc alpha, anti-pPKAc (T197), anti-GSK-3α/β, anti-pGSK-3α/β (S21/9) (Cell Signaling), anti-PP1, anti-c-src, anti-p-c-src (Y419), anti-E1A protein (Santa Cruz Biotechnology), anti-phospho PPP1A (T320), anti-PP2Aα/β (Abcam), and anti-fiber protein (Neomarkers). Electrophoresis reagents were purchased from Bio-rad and trypsin from Promega.
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