Malvern mastersizer 2000e analyzer

The particle size distribution (PSD) of initial and stored SB was determined in the diameter range of 0.1e1000 mm by laser diffraction using a Malvern Mastersizer 2000E analyzer (Malvern Instruments Ltd; Worcestershire, UK). PSD were expressed as volume frequency (Â100). Optical parameters applied were: relative refractive index, 1.14 and adsorption coefficient: 0.01. Before particle size measurements, samples were carefully mixed by turning the containers upside down to get a particle size for the whole sample. These aliquots were then dispersed in 600 ml of water at 2000 rpm in the dispersion unit (Hydro 2000 MU, Malvern Instruments Ltd; Worcestershire, UK). From PSD, the De Brouckere (volume-weighted, D 4,3 ) moment mean diameter was obtained.
From the obtained data, the particle size increase (PSI) was calculated as follows:
where D 0 is the initial D 4,3 value and D f is the D 4,3 value after frozen storage for 30 days.

The particle size distributions were determined by laser diffraction using a Malvern Mastersizer 2000E analyzer (Malvern Instruments, Malvern, UK) associated with a wet dispersion unit (Hydro 2000MU, Malvern Instruments, Malvern, UK). The pump speed was fixed at 2000 rpm. Mean Sauter diameters (d 3,2 ) and mean De Brouckere diameters (d 4,3 ) were determined immediately after emulsion formation and after 24 h of storage at 20 °C.

The effect of the isolation conditions on the particle size distribution of CPI was studied by laser diffraction, using a Malvern Mastersizer 2000E analyzer (Malvern Instruments, Malvern, UK). A wet dispersion unit was associated (Hydro 2000MU, Malvern Instruments, Malvern, UK), setting the pump speed at 2000 rpm. Dispersions from CPI were prepared at 30 g/L, in buffer Tris-HCl 100 mmol/L. Particles were assumed to have a relative refractive index of 1.4 and the adsorption coefficient was fixed at 0.1.