Paclitaxel was obtained from National Institute for Food and Drug Control. Soybean phospholipids (SPC) was purchased from Kelong Chemical (Chengdu, China). Cholesterol (Chol) was purchased from Bio Life Science & Technology Co., Ltd (Shanghai, China). D-(+)-Biotin was purchased from shanghai darui fine chemical Co., Ltd (Shanghai, China). GOx was obtained from Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-snglycero-3-phosphoethanolamine-N-(carboxyfluorescein) (CFPE) was purchased from Avanti Polar Lipids (USA). 4'-6-Diamidino-2-phenylindole (DAPI) and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Beyotime Institute Biotechnology (Haimen, China). 4-chlorobenzenesulfonate salt (DiD) was purchased from Biotium (USA). Annexin V-FITC/PI apoptosis detection kit was obtained from BD PharmingenTM (USA). Enhanced BCA Protein Assay Kit (Beyotime®, China). Hydrogen Peroxide (H2O2) Assay Kit (Beyotime®, China). Reactive Oxygen Species (ROS) Assay Kit (Beyotime®, China). Calcein-AM/PI Live/Dead Cell Double Dyed Kit (Solarbio®, China). JC-1 dye (Beyotime®, China).
1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein cfpe
Manufactured by Avanti Polar Lipids
Sourced in United States
1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein (CFPE) is a synthetic phospholipid compound used in various laboratory applications. It contains a fluorescent carboxyfluorescein group attached to the head group of the phospholipid. CFPE can be utilized as a fluorescent marker or tracer in biological studies, but its specific applications and intended uses are not provided in this factual description.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
4 chlorobenzenesulfonate salt did, by Biotium (1 mentions)
Hydrogen peroxide h2o2 assay kit, by Beyotime (1 mentions)
Jc 1 dye, by Beyotime (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by BD (1 mentions)
Enhanced bca protein assay kit, by Beyotime (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Beyotime (1 mentions)
Reactive oxygen species assay kit, by Beyotime (1 mentions)
Ros detection kit, by Beyotime (1 mentions)
Bend 3, by American Type Culture Collection (1 mentions)
Reverse transcription system kit, by Thermo Fisher Scientific (1 mentions)
Stempro accutase cell dissociation reagent, by Thermo Fisher Scientific (1 mentions)
Insulin transferrin selenium, by Thermo Fisher Scientific (1 mentions)
N hydroxysuccinimide nhs, by Merck Group (1 mentions)
Cd133 microbead kit, by Miltenyi Biotec (1 mentions)
Gefitinib, by Meilun (1 mentions)
Lmp agarose, by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine 4 chlorobenzenesulfonate salt did, by Biotium (1 mentions)
Egg phosphatidylcholine epc, by Avanti Polar Lipids (1 mentions)
6 protocols using 1 2 dioleoyl sn glycero 3 phosphoethanolamine n carboxyfluorescein cfpe
1
Paclitaxel-Loaded Lipid Nanoparticles for Cancer Therapy
2
Quercetin-Loaded Lipid Nanoparticles for Neuroprotection
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All unspecified reagents were from commercial resources. Quercetin (QU) was obtained from Sichuan Academy of Agriculture Sciences (Chengdu, China). Soybean phospholipids (SPC, majorly with phosphatidylserine, and other components) and cholesterol (Chol) were obtained from Kelong Chemical (Chengdu, China). 4′-6-Diamidino-2-phenylindole (DAPI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and ROS detection kit were purchased from Beyotime Institute Biotechnology (Haimen, China). 1,2-Dioleoyl-snglycero-3-phosphoethanolamine-N-(carboxyfluorescein) (CFPE) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fetal bovine serum (FBS) was purchased from Gibico (Oakland, CA, USA). PC12 (rat adrenal pheochromocytoma cells) and bEnd.3 (mouse brain microvascular endothelial cells) cells were purchased from American Type Culture Collection. All other reagents were of analytical grade.
3
Magnetic CD133+ Cell Isolation Protocol
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1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] (DSPE-PEG2000-Mal), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein (CFPE) were bought from Avanti Polar Lipids (Alabaster, AL). Phycoerythrin-labeled CD133 antibodies and the CD133 MicroBead Kit were bought from Miltenyi Biotec (Auburn, CA). Gefitinib was bought from Dalian Meilun Biotech (Dalian, China). The primers and thiolated CD133 aptamer with a sequence of 5′-SH-CCCUCCUACAUAGGG-3′ were bought from Ruibo Co., Ltd. (Guangzhou, China). N-Hydroxysuccinimide (NHS), 1-ethyl-3-3-dimethylaminopropyl carbodiimide (EDC), epidermal growth factor (EGF), and basic fibroblast growth factor (bFGF) were bought from Sigma-Aldrich (St. Louis, MO). The StemPro® Accutase® Cell Dissociation Reagent, the Reverse Transcription System kit, TRIzol® Reagent, B27, and insulin-transferrin-selenium (ITS) were bought from Thermo Fisher Scientific Inc. All organic reagents (analytical grade) were provided by Sinopharm (Shanghai, China) unless otherwise stated.
4
Cholesterol-Anchored Functional Nanomaterials
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Egg phosphatidylcholine (EPC) and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(carboxyfluorescein) (CFPE) were purchased from Avanti Polar Lipids, Inc. (Alabaster, USA). Cholesterol (CHO) and cysteine (Cys) were purchased from Chengdu Kelong Chemical Company (Chengdu, China). All the cholesterol anchored functional materials were synthesized by our laboratory according to a previously reported method17 , including the cholesterol anchored reduction-sensitive PEG (CHO-S-S-PEG5000), R8 modified PEG (CHO-PEG2000-R8) and non-cleavable PEG2000 (CHO-mPEG2000). Agarose LMP was purchased from Invitrogen (Carlsbad, CA). 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine 4-chlorobenzenesulfonate salt (DID) was obtained from Biotium (Hayward, CA). Other chemicals and reagents were of analytical grade.
Male BALB/c mice weighing 20–25 g were purchased from Experiment Animal Center of Sichuan University (China). All the animal experiments adhered to the principles of care and use of laboratory animals and were approved by the Experiment Animal Administrative Committee of Sichuan University.
Male BALB/c mice weighing 20–25 g were purchased from Experiment Animal Center of Sichuan University (China). All the animal experiments adhered to the principles of care and use of laboratory animals and were approved by the Experiment Animal Administrative Committee of Sichuan University.
5
Salinomycin-Loaded Targeted PLGA Nanoparticles
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Salinomycin sodium, poly(d, l-lactide-co-glycolide) (PLGA, 50:50, Mw 40,000-75,000 Da), was purchased from Sigma-Aldrich (St Louis, MO, USA). Soybean lecithin was obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy(polyethylene glycol)-2000) (DSPE-PEG2000), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(maleimide(polyethylene glycol)-2000) (DSPE-PEG2000-Mal), and 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-carboxyfluorescein (CFPE) were obtained from Avanti Polar Lipids (Alabaster, USA). The EGFR-targeted peptide GE11, CGGGSYHWYGYTPQNVI, was synthesized by Apeptide Ltd. (Shanghai, China), with the “GGGS” sequence as a spacer and the N-terminal amino cysteine of the peptide reacting with the maleimide group of Sal-NPs. Cell Counting Kit-8 (CCK-8) was obtained from Dojindo (Kumamoto, Japan). All other chemicals were of analytical grade.
6
Lipid-Based Nanoparticle Delivery System for siRNA Therapy
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Cholesterol was purchased from Chengdu Kelong Chemical Company (Chengdu, China). Soybean phospholipids (SPC) were purchased from Shanghai Taiwei Chemical Company (Shanghai, China). 1, 2-dioleoyl-3-trimethylammonium-propane (DOTAP) was obtained from Shanghai Advanced Vehicle Technology (AVT) Ltd. (Shanghai, China). DSPE-PEG2000-OMe and 1, 2-dioleoyl-sn-glycero-3- phosp-hoethanolamine-N-(carboxyfluorescein) (CFPE ) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Protamine sulfate (fraction X from salmon) and calf thymus DNA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paclitaxel (PTX) and low-molecular-weight heparin (Enoxaparin sodium, LH) were purchased from Melonepharma (Dalian, China). BCL-2 siRNA, scrambled siRNA and Cy5-labeled siRNA were purchased from Gene Pharma Ltd. (Shanghai, China). The BCL-2 siRNA (siBCL-2) sense sequence is 5'-GUG AUG AAG UAC AUC CAU UdTdT-3', and the anti-sense sequence is 5'-AAU GGA UGU ACU UCA UCA CdTdT-3'. The scramble siRNA (siCTL) sense sequence is 5'-UUC UCC GAA CGU GUC ACG UTT-3', and the anti-sense sequence is 5'-ACG UGA CAC GUU CGG AGA ATT-3'.
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