GalT-KO donor swine were anesthetized with I.M. Telazol 2 mg/kg, intubated, and had anesthesia maintained with 2% isoflurane and oxygen. Presurgery, the area of donor skin was disinfected with 2% chlorhexidine acetate (NolvasanR Surgical Scrub, Fort Dodge Animal Health, Fort Dodge, IA), 70% isopropyl rubbing alcohol (NolvasanR Surgical Scrub, Owens & Minor, Mechanicsville, VA), and 10% povidone-iodine (Betadine Solution, Purdue Products, L.P., Stamford, CT) before sterile draping. An air-driven Zimmer dermatome (Medfix Solution, Inc., Tucson, AZ) with a depth of 0.022 inches was used to harvest split-thickness skin grafts (STSGs) from the dorsum of the animal. Saline and epinephrine soaked gauze was applied to skin donor sites for hemostasis before dressing with Telfa™ (Covidien, Dublin, Ireland) and Tegaderm™ (3M, St. Paul, MN).
Baboon donors were sedated with I.M. atropine 0.1 mg/kg and I.M. ketamine 20 mg/kg, shaved, and transferred to the operating room for endotracheal intubation and general anesthesia with 2% isoflurane and oxygen similar to the above. Skin donor sites on the baboon’s back was prepped as described above for GalT-KO swine.
Baboon donors were sedated with I.M. atropine 0.1 mg/kg and I.M. ketamine 20 mg/kg, shaved, and transferred to the operating room for endotracheal intubation and general anesthesia with 2% isoflurane and oxygen similar to the above. Skin donor sites on the baboon’s back was prepped as described above for GalT-KO swine.