IOMM-Lee and HKBMM, human malignant meningioma cell lines, were obtained from the American Type Culture Collection (Manassas, VA, USA) and from the Riken BioResource Center (Tsukuba, Japan), respectively. IOMM-Lee was cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). HKBMM was cultured in Ham’s F12 medium supplemented with 10% FBS.
Hkbmm
Manufactured by American Type Culture Collection
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The HKBMM is a laboratory equipment product offered by the American Type Culture Collection. It serves as a core function for the culturing and handling of various biological samples and materials.
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3 protocols using hkbmm
1
Culturing Human Malignant Meningioma Cells
2
Malignant Meningioma Cell Lines
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IOMM-Lee and HKBMM, human malignant meningioma cell lines, were obtained from the American Type Culture Collection (Manassas, VA, USA) and from the Riken BioResource Center (Tsukuba, Japan), respectively. Their characteristics as malignant meningioma cells were confirmed in a previous study [8 (link)]. IOMM-Lee cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS). HKBMM cells were cultured in Ham’s F12 medium supplemented with 10% FBS.
3
Malignant Meningioma Cell Lines: Protocols
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The malignant meningioma cell lines IOMM‐Lee and HKBMM (Figure S1 ) were obtained from the American Type Culture Collection (ATCC; #CRL‐3370) and RIKEN cell bank (RIKEN BioResource Research Center; #RCB0680), respectively. The details of the cell culture conditions and gene mutation status are presented in the supporting document (Doc S1 ). The RdRP activity status of IOMM‐Lee and HKBMM cells are illustrated in Figure S2 .
The details of the cell viability assay, flow cytometric cell cycle analysis, immunoblotting, tubulin polymerization assay, siRNA‐mediated TERT knockdown, real‐time PCR, dye exclusion assay, migration assay, animal experiments, immunohistochemistry, immunoprecipitation‐RdRP assay, and statistical analysis are provided as supporting information (DocS1 ).
The details of the cell viability assay, flow cytometric cell cycle analysis, immunoblotting, tubulin polymerization assay, siRNA‐mediated TERT knockdown, real‐time PCR, dye exclusion assay, migration assay, animal experiments, immunohistochemistry, immunoprecipitation‐RdRP assay, and statistical analysis are provided as supporting information (Doc
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